Studies on the photobiological activity of two naturally occurring furochromones, visnagin and khellin, in Chlamydomonas reinhardtii

Irradiation of arg-1 cells of the green alga Chlamydomonas reinhardtii with UV-A in the presence of visnagin (10 μg/ml)produced weak mutagenic effects when a fluence rate of 5.1 W/m2 and fluences of 1.5-36.7 kJ/m2 were applied. A maximum number of revertants was obtained at ˜9.2 kJ/m2. When a fluenc...

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Bibliographic Details
Published in:Mutagenesis 1997-05, Vol.12 (3), p.141-145
Main Author: SCHIMMER, O
Format: Article
Language:English
Subjects:
5-Methoxypsoralen
Animals
Biological and medical sciences
Chemical mutagenesis
Chlamydomonas reinhardtii
Chlamydomonas reinhardtii - drug effects
Chlamydomonas reinhardtii - genetics
Chlamydomonas reinhardtii - radiation effects
Dose-Response Relationship, Radiation
Freshwater
Khellin - analogs & derivatives
Khellin - toxicity
Medical sciences
Methoxsalen - analogs & derivatives
Methoxsalen - toxicity
Mutagenicity Tests
Mutagens - toxicity
Photobiology
Photosensitizing Agents - toxicity
Toxicology
Ultraviolet Rays - adverse effects
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Summary:Irradiation of arg-1 cells of the green alga Chlamydomonas reinhardtii with UV-A in the presence of visnagin (10 μg/ml)produced weak mutagenic effects when a fluence rate of 5.1 W/m2 and fluences of 1.5-36.7 kJ/m2 were applied. A maximum number of revertants was obtained at ˜9.2 kJ/m2. When a fluence rate of 20.4 Wm2 was used the photomutagenicity of visnagin was markedly enhanced with fluences of 3=36.7 kJ/m2. In survival experiments with a fluence rate of 5.1 W/m2 the surviving fraction decreased continuously to ˜4%. In experiments with a fluence rate of 20.4 W/m2, however, higher survival rates were observed compared at equal UV-A doses. Visnagin was much less phototoxic and photomutagenic than bergapten whencompared at equimolar concentrations and equal UV-A doses. Re-irradiation with UV-A in the absence of unbound visnagin did not alter survival and mutagenicity which had been induced by the first treatment. The mutation frequency plotted versus the UV-A fluence exhibited secondorder kinetics. Khellin showed only marginal photosensitizing capacity and no significant mutagenicity up to a concentration of 100 μg/ml and a total UV-A fluence of 73.4 kJ/m2.
ISSN:0267-8357
1464-3804
DOI:10.1093/mutage/12.3.141