BICARBONATE UTILIZATION BY MARINE PHYTOPLANKTON SPECIES

ABSTRACT The contribution of bicarbonate to total dissolved inorganic carbon (DIC) utilization was investigated using 18 marine phytoplankton species, including members of Bacillariophyceae, Dinophyceae, Prymnesiophyceae, and Raphidophyceae, under carbon‐replete or ‐limited conditions. Extracellular...

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Bibliographic Details
Published in:Journal of phycology 1997-08, Vol.33 (4), p.625-631
Main Authors: Nimer, Nabil A., Iglesias-Rodriguez, M. Debora, Merrett, Michael J.
Format: Article
Language:English
Subjects:
2-disulfonic acid (DIDS)
4′4′-diisothiocyanatostilbene-2
4′4′‐diisothiocyanatostilbene‐2,2‐disulfonic acid (DIDS)
Bacillariophyceae
bicarbonate utilization: dextran-bound sulfonamide (DBS)
Chaetoceros compressus
Chrysochromulina kappa
Dinophyceae
Dinophyta
extracellular carbonic anhydrase
Glenodinium foliaceum
Key index words: alkalization
Marine
phytoplankton
Prymnesiophyceae
Raphidophyceae
Thalassiosira pseudonana
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Summary:ABSTRACT The contribution of bicarbonate to total dissolved inorganic carbon (DIC) utilization was investigated using 18 marine phytoplankton species, including members of Bacillariophyceae, Dinophyceae, Prymnesiophyceae, and Raphidophyceae, under carbon‐replete or ‐limited conditions. Extracellular carbonic anhydrase (CA) was assayed as an indicator of extracellular CA‐catalyzed HCO−3 utilization. For some species, extracellular CA was constitutive, in others activity was detected under conditions of carbon limitation, and in others, even under carbon‐limited conditions, activity was not detected. In species without extracellular CA, direct HCO−3 uptake was investigated using a pH drift technique in a closed system, DIC measurements, and the use of the anion exchange inhibitor 4′4′‐diisothiocyanatostilbene‐2,2‐disulfonic acid (DLDS). Three of these species (Chaetoceros compressus, Thalassiosira pseudonana, and Glenodinium foliaceum) gave a pH drift not inhibited by DIDS, but cultures of Chrysochromulina kappa, Gephrocapsa oceanica, and Coccolithus pelagicus, in which DLDS inhibited DIC uptake, did not give a pH drift. This result shows that direct HCO3− transport may occur by an anion exchange‐type mechanism in some species but not others. Of the eighteen species investigated, only Heterosigma akashiwo did not have the potential for direct uptake or extracellular CA‐catalyzed HCO−3 utilization.
ISSN:0022-3646
1529-8817
DOI:10.1111/j.0022-3646.1997.00625.x