Metabolic activation of aromatic amines by human pancreas

Epidemiologic studies have suggested that aromatic amines (and nitroaromatic hydrocarbons) may be carcinogenic for human pancreas. Pancreatic tissues from 29 organ donors (13 smokers, 16 non-smokers) were examined for their ability to metabolize aromatic amines and other carcinogens. Microsomes show...

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Bibliographic Details
Published in:Carcinogenesis (New York) 1997-05, Vol.18 (5), p.1085-1092
Main Authors: ANDERSON, K. E, HAMMONS, G. J, GUENGERICH, F. P, BARONE, G. W, LANG, N. P, PETERSON, L. A, KADLUBAR, F. F, POTTER, J. D, KADERLIK, K. R, ILETT, K. F, MINCHIN, R. F, TEITEL, C. H, CHOU, H.-C, MARTIN, M. V
Format: Article
Language:English
Subjects:
Acetyltransferases - metabolism
Amines - metabolism
Biological and medical sciences
Biotransformation
Biphenyl Compounds - metabolism
Carcinogenesis, carcinogens and anticarcinogens
Chemical agents
Cytosol - metabolism
Female
Humans
Male
Medical sciences
Microsomes - metabolism
Nitrosamines - metabolism
Oxidation-Reduction
Pancreas - metabolism
Smoking
Tumors
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Summary:Epidemiologic studies have suggested that aromatic amines (and nitroaromatic hydrocarbons) may be carcinogenic for human pancreas. Pancreatic tissues from 29 organ donors (13 smokers, 16 non-smokers) were examined for their ability to metabolize aromatic amines and other carcinogens. Microsomes showed no activity for cytochrome P450 (P450) 1A2-dependent N-oxidation of 4-aminobiphenyl (ABP) or for the following activities (and associated P450s): aminopyrine N-demethylation and ethylmorphine N-demethylation (P450 3A4); ethoxyresorufin O-deethylation (P450 1A1) and pentoxyresorufin O-dealkylation (P450 2B6); p-nitrophenol hydroxylation and N-nitrosodimethyl-amine N-demethylation (P450 2E1); lauric acid omega-hydroxylation (P450 4A1); and 4-(methylnitrosamino)-1-(3-pyridyl-1-butanol) (NNAL) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) alpha-oxidation (P450 1A2, 2A6, 2D6). Antibodies were used to examine microsomal levels of P450 1A2, 2A6, 2C8/9/18/19, 2E1, 2D6, and 3A3/4/5/7 and epoxide hydrolase. Immunoblots detected only epoxide hydrolase at low levels; P450 levels were
ISSN:0143-3334
1460-2180
1460-2180
DOI:10.1093/carcin/18.5.1085