Allelochemicals produced during glucosinolate degradation in soil

• Published in: Journal of chemical ecology 1991-10, Vol.17 (10), p.2021-2034
• Main Authors: BROWN, P. D, MORRA, M. J, MCCAFFREY, J. P, AULD, D. L, WILLIAMS, L. III
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biochemistry and biology; Biological and medical sciences; Brassica napus; Chemical, physicochemical, biochemical and biological properties; Fundamental and applied biological sciences. Psychology; Generalities; Invertebrates; Physics, chemistry, biochemistry and biology of agricultural and forest soils; Soil science
• ISSN: 0098-0331; 1573-1561
• DOI: 10.1007/BF00992585

A variety of plant pests are suppressed by the incorporation of cruciferous plant material into soil. Although this effect is attributed to decomposition of glucosinolates into toxic products, little is known concerning glucosinolate degradation in the soil environment. Arenas (30 × 18 × 8 cm) that contained soil amended with 30 g defatted winter rapeseed meal (Brassica napus L.)/kg soil on one half and unamended soil on the other were constructed. Isothiocyanate concentrations in the soil were measured using infrared analysis of CC14 extracts, and ionic thiocyanate (SCN(-)) using ion chromatography on aqueous extracts. Quantities were monitored during a 100-hr time period in conjunction with a wireworm bioassay. Isothiocyanate production reached a maximum of 301 nmol/g soil at 2 hr, but decreased by 90% within 24 hr. Production of SCN(-) reached a maximum of 180 nmol/g soil at 8 hr but persisted longer than isothiocyanate. Separate late instar wire-worms (Limonius infuscatus Mots.) were repelled by the presence of rapeseed meal in less than 24 hr even though the meal was shown in separate experiments not to be toxic. We propose that rapidly produced isothiocyanates are responsible for this repellency, but other products such as SCN(-) may play a role.