The promoter of the gene encoding the C sub(4) form of phosphoenolpyruvate carboxylase directs mesophyll-specific expression in transgenic C sub(4) Flaveria spp

The function of the C sub(4) mechanism of photosynthesis depends on the strict compartmentation of the enzymes involved. Here, we investigate the regulatory mechanisms that ensure the mesophyll-specific expression of the C sub(4) isoform of phosphoenolpyruvate carboxylase. We show that 2 kb of the 5...

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Bibliographic Details
Published in:The Plant cell 1997-04, Vol.9 (4), p.479-489
Main Authors: Stockhaus, J, Schlue, U, Koczor, M, Chitty, JA, Taylor, W C, Westhoff, P
Format: Article
Language:English
Subjects:
Flaveria bidentis
Flaveria trinervia
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Summary:The function of the C sub(4) mechanism of photosynthesis depends on the strict compartmentation of the enzymes involved. Here, we investigate the regulatory mechanisms that ensure the mesophyll-specific expression of the C sub(4) isoform of phosphoenolpyruvate carboxylase. We show that 2 kb of the 5' flanking region of the Flaveria trinervia C sub(4) PpcA1 gene is sufficient to direct mesophyll-specific expression of the beta -glucuronidase reporter gene in transgenic F. bidentis (C sub(4)) plants. In young leaves of seedlings, the activity of this promoter is dependent on the developmental stage of the mesophyll cells. It is induced in a basipetal fashion (leaf tip to base) during leaf development. The promoter region of the orthologous nonphotosynthetic Ppc gene of F. pringlei (C sub(3)) induces reporter gene expression mainly in the vascular tissue of leaves and stems as well as in mesophyll cells of transgenic F. bidentis plants. Our experiments demonstrate that during the evolution of the C sub(4) Flaveria species, cis-acting elements of the C sub(4) Ppc gene must have been altered to achieve mesophyll-specific expression.
ISSN:1040-4651