Rejection and Continuous Regeneration of the Native Coenzyme NAD(P)H in a Charged Ultrafiltration Membrane Enzyme Reactor

A method has been developed that allows use of dehydrogenases in continuous processes. Results on two of these systems are presented here. In one, mannitol is produced from fructose by mannitol dehydrogenase (MDH) and NADH is regenerated simultaneously by glucose dehydrogenase (GDH) from Bacillus me...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 1990-01, Vol.613 (1), p.820-826
Main Authors: KULBE, KLAUS D., HOWALDT, MICHAEL W., SCHMIDT, KARLHEINZ, RÖTHIG, THOMAS R., CHMIEL, HORST
Format: Article
Language:English
Subjects:
alcohol dehydrogenase
bioreactors
glucose dehydrogenase
mannitol dehydrogenase
NADH
Thermoanaerobium brockii
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Summary:A method has been developed that allows use of dehydrogenases in continuous processes. Results on two of these systems are presented here. In one, mannitol is produced from fructose by mannitol dehydrogenase (MDH) and NADH is regenerated simultaneously by glucose dehydrogenase (GDH) from Bacillus megaterium , which converts glucose to gluconic acid. Both products are used in the food industry. In the other process, the enantiomeric alcohol S-sulcatol, a pheromone of a bark beetle, is produced together with a ketone by using alcohol dehydrogenase from Thermoanaerobium brockii (TBADH).
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.1990.tb18271.x