Overexpression of glutathione S-transferase/glutathione peroxidase enhances the growth of transgenic tobacco seedlings during stress

Transgenic tobacco seedlings that overexpress a cDNA encoding an enzyme with both glutathione S-transferase (GST) and glutathione peroxidase (GPX) activity had GST- and GPX-specific activities approximately twofold higher than wild-type seedlings. These GST/GPX overexpressing seedlings grew signific...

Full description

Saved in:
Bibliographic Details
Published in:Nature biotechnology 1997-10, Vol.15 (10), p.988-991
Main Authors: Roxas, V.P. (Texas Tech University, Lubbock, TX.), Smith, R.K. Jr, Allen, E.R, Allen, R.D
Format: Article
Language:English
Subjects:
Adaptation to environment and cultivation conditions
Agriculture
Agronomy. Soil science and plant productions
Biological and medical sciences
Biotechnology
COLD
Cold Temperature
COOLING
DNA, Complementary - genetics
ENFRIAMIENTO
Escherichia coli - enzymology
Escherichia coli - genetics
FRIO
FROID
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Enzymologic - genetics
Genetic engineering
Genetic technics
Genetics and breeding of economic plants
Glutathione Peroxidase - biosynthesis
Glutathione Peroxidase - genetics
Glutathione Transferase - biosynthesis
Glutathione Transferase - genetics
Methods. Procedures. Technologies
Nicotiana - genetics
Nicotiana - growth & development
NICOTIANA TABACUM
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants, Genetically Modified
Plants, Toxic
REFROIDISSEMENT
RESISTANCE A LA TEMPERATURE
RESISTENCIA A LA TEMPERATURA
SALINIDAD
SALINITE
SALINITY
SALT TOLERANCE
TEMPERATURE RESISTANCE
TOLERANCE AU SEL
TOLERANCIA A LA SAL
Transgenic animals and transgenic plants
TRANSGENIC PLANTS
Varietal selection. Specialized plant breeding, plant breeding aims
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Transgenic tobacco seedlings that overexpress a cDNA encoding an enzyme with both glutathione S-transferase (GST) and glutathione peroxidase (GPX) activity had GST- and GPX-specific activities approximately twofold higher than wild-type seedlings. These GST/GPX overexpressing seedlings grew significantly faster than control seedlings when exposed to chilling or salt stress. During chilling stress, levels of oxidized glutathione (GSSG) were significantly higher in transgenic seedlings than in wild-types. Growth of wild-type seedlings was accelerated by treatment with GSSG, while treatment with reduced glutathione or other sulfhydryl-reducing agents inhibited growth. Therefore, overexpression of GST/GPX can stimulate seedling growth under chilling and salt stress, and this effect could be caused by oxidation of the glutathione pool.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt1097-988