Isolation and characterization of intergeneric somatic hybrids in the Apocynaceae family

Protoplasts were isolated from callus cultures of Rauwolfia serpentina Benth., Rhazya stricta Decaisne, and Catharanthus roseus (L.) G. Don, or from leaves of Vinca minor L. Protoplast isolation, culture, and fusion techniques as well as hybrid screening systems were developed for these species, and...

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Bibliographic Details
Published in:Theoretical and applied genetics 1991-10, Vol.82 (6), p.713-716
Main Authors: Kostenyuk, I. (Inst. of Cell Biology and Genetic Engineering, Kiev (Russian Federation)), Lubaretz, O, Borisyuk, N, Voronin, V, Stoeckigt, J, Gleba, Y
Format: Article
Language:English
Subjects:
ADN
ALCALOIDE
ALCALOIDES
ALKALOIDS
APOCYNACEAE
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
DNA
Fundamental and applied biological sciences. Psychology
FUSION DE PROTOPLASTES
FUSION DEL PROTOPLASTO
Genetics of eukaryotes. Biological and molecular evolution
HIBRIDACION SOMATICA
HYBRIDATION SOMATIQUE
INDOL
Indole alkaloid
INDOLES
ISOENZIMAS
ISOENZYME
ISOENZYMES
Isozyme spectrum
PROTOPLAST FUSION
Pteridophyta, spermatophyta
Ribosomal DNA
RIBOSOMAS
RIBOSOME
RIBOSOMES
Somatic hybrid
SOMATIC HYBRIDIZATION
Vegetals
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Summary:Protoplasts were isolated from callus cultures of Rauwolfia serpentina Benth., Rhazya stricta Decaisne, and Catharanthus roseus (L.) G. Don, or from leaves of Vinca minor L. Protoplast isolation, culture, and fusion techniques as well as hybrid screening systems were developed for these species, and hybrids were obtained. Hybrid combinations were Rauwolfia + Vinca, Rauwolfia + Catharanthus, Rauwolfia + Rhazya, and Catharanthus + Vinca. For hybrid isolation, the physiological complementation method was utilized. Analyses of the material obtained included a cytogenetic study of the chromosomes, a study of multiple molecular forms of transferases and esterases, and the blot hybridization of restricted nuclear DNA using ribosomal DNA as a probe. Hybrids were identified in all species' combinations tried. A ten-fold increase in the accumulation of raucaffricine (relative to the parental Rauwolfia strain) was observed in one cell line of the Rauwolfia + Vinca hybrid. Our studies indicated the genetic stability of the great majority of the hybrid cell lines over a period of more than 20 months of in vitro growth. No shoot morphogenesis has so far been observed in this material.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00227315