Effect of complex nitrogen sources on the production of penicillin acylase by bacillus megaterium

The following complex nitrogen sources were evaluated for the production of penicillin acylase by Bacillus megaterium: casein hydrolysate, corn steep liquor, stick water concentrate, blood meal and defatted sunflower meal. Experiments were run in shake flasks at 30 degree C and pH 7.0. Best results...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 1997, Vol.13 (1), p.127-128
Main Authors: GENTINA, J. C, ACEVEDO, F, VILLAGRA, M. P
Format: Article
Language:English
Subjects:
Bacillus megaterium
Biological and medical sciences
Biotechnology
Enzyme engineering
Fermentation
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Production of selected enzymes
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Summary:The following complex nitrogen sources were evaluated for the production of penicillin acylase by Bacillus megaterium: casein hydrolysate, corn steep liquor, stick water concentrate, blood meal and defatted sunflower meal. Experiments were run in shake flasks at 30 degree C and pH 7.0. Best results were obtained with casein hydrolysate: 244 IU/l were produced with a productivity of 20.3 IU /l/h and yield of 717.6 IU/g of nitrogen. The lowest results correspond to sunflower meal with 39 IU/l.
ISSN:0959-3993
1573-0972
DOI:10.1007/BF02770818