Differences between, and possible origins of, the cytoplasms found in fertile and male-sterile onions (Allium cepa L.)

The DNA of the organellar genomes of Allium cepa has been examined to detect restriction fragment length polymorphisms. Differences can be shown between both the chloroplastal and mitochondrial genomes of the N and cms-S cytoplasms in their restriction fragment profiles. Southern blot analysis of th...

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Bibliographic Details
Published in:Theoretical and applied genetics 1991-10, Vol.82 (6), p.737-744
Main Authors: Holford, P. (Birmingham Univ. (United Kingdom). School of Biological Sciences), Croft, J.H, Newbury, H.J
Format: Article
Language:English
Subjects:
ADN
ALLIUM CEPA
Biological and medical sciences
Chloroplastal DNA
CHLOROPLASTE
CHLOROPLASTS
Classical genetics, quantitative genetics, hybrids
CLOROPLASTO
CYTOPLASMIC MALE STERILITY
DNA
ENZIMA DE RESTRICCION
ENZYME DE RESTRICTION
ESTERILIDAD MASCULINA CITOPLASMICA
Fundamental and applied biological sciences. Psychology
GENETIC POLYMORPHISM
GENETICA MITOCHONDRIAL
Genetics of eukaryotes. Biological and molecular evolution
GENETIQUE MITOCHONDRIALE
Mitochondrial DNA
MITOCHONDRIAL GENETICS
NUCLEOTIDE SEQUENCE
POLIMORFISMO GENETICO
POLYMORPHISME GENETIQUE
Pteridophyta, spermatophyta
RESTRICTION ENZYMES
Restriction fragment length polymorphism
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
STERILITE MALE CYTOPLASMIQUE
Vegetals
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Summary:The DNA of the organellar genomes of Allium cepa has been examined to detect restriction fragment length polymorphisms. Differences can be shown between both the chloroplastal and mitochondrial genomes of the N and cms-S cytoplasms in their restriction fragment profiles. Southern blot analysis of the mtDNA profiles using probes containing defined mitochondrial genes also detected polymorphisms. No differences can be shown between the organellar genomes of the N and cms-T onions by either of these techniques. These data indicate different origins for the two sterility-conferring cytoplasms, suggesting autoplasmic and alloplasmic origins for the cms-T and cms-S cytoplasms, respectively. No evidence of the presence of virus-like particles was found in any of the cytoplasms.
ISSN:0040-5752
1432-2242
DOI:10.1007/bf00227319