Localization of the human tissue factor recognition determinant of human factor VIIa

Tissue factor is an integral membrane glycoprotein that serves as an essential cofactor for the blood coagulation factor VIIa. Recent studies have attempted to localize the tissue factor recognition determinant of human factor VIIa. While several regions of factor VIIa have been implicated as import...

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Bibliographic Details
Published in:The Journal of biological chemistry 1991-10, Vol.266 (29), p.19198-19202
Main Authors: TOOMEY, J. R, SMITH, K. J, STAFFORD, D. W
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Blood coagulation. Blood cells
Chimera
Cloning, Molecular
Coagulation factors
DNA - genetics
Electrophoresis, Polyacrylamide Gel
Epidermal Growth Factor - genetics
Factor VIIa - genetics
Factor VIIa - metabolism
Fundamental and applied biological sciences. Psychology
Humans
Molecular and cellular biology
Molecular Sequence Data
Mutagenesis
Polymerase Chain Reaction
Thromboplastin - metabolism
tissue factor
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Summary:Tissue factor is an integral membrane glycoprotein that serves as an essential cofactor for the blood coagulation factor VIIa. Recent studies have attempted to localize the tissue factor recognition determinant of human factor VIIa. While several regions of factor VIIa have been implicated as important for tissue factor binding, the high affinity tissue factor recognition determinant of human factor VIIa is unknown. In order to define the determinant, we constructed a set of six chimeric proteins composed of portions of factor VII and factor IX. We then utilized the chimeras in competition experiments with 125I-labeled factor VIIa for recombinant tissue factor bound to an Immobilon-P membrane. The data indicate that the high affinity tissue factor recognition determinant of human factor VIIa is within the epidermal growth factor domains.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)54982-6