Determination of the polyester polyurethane breakdown products and distribution of the polyurethane degrading enzyme of Comamonas acidovorans strain TB-35

Metabolites produced by the degradation of polyester polyurethane (PUR) by Comamonas acidovorans strain TB-35 were investigated. GC-MS analysis revealed that they were diethylene glycol, trimethylolpropane and adipic acid. These metabolites were considered to be derived from polyester segments of th...

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Bibliographic Details
Published in:Journal of fermentation and bioengineering 1997-01, Vol.83 (5), p.456-460
Main Authors: Nakajima-Kambe, Toshiaki, Onuma, Fumiko, Akutsu, Yukie, Nakahara, Tadaatsu
Format: Article
Language:English
Subjects:
BACTERIA
Bacteriology
BIODEGRADACION
BIODEGRADATION
Biological and medical sciences
Comamonas acidovorans
ESTERASAS
ESTERASE
ESTERASES
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Microbiology
POLIURETANO
POLYURETHANE
polyurethane degradation
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Summary:Metabolites produced by the degradation of polyester polyurethane (PUR) by Comamonas acidovorans strain TB-35 were investigated. GC-MS analysis revealed that they were diethylene glycol, trimethylolpropane and adipic acid. These metabolites were considered to be derived from polyester segments of the PUR as a result of hydrolytic cleavage of ester bonds. In addition, when the culture broth was alkaline treated, a previously undetected product was detected by GC analysis. This product was identified as 2,4-diaminotoluene by GC-MS analysis. This indicates that an additional metabolite exists in the culture broth. This metabolite was considered to have been derived from polyisocyanate segments of the PUR. A preliminary study on a PUR degrading enzyme was also performed. Strain TB-35 produced two different esterases, one which is secreted to the culture broth and one which is bound to the cell surface. Between them, only cell-surface-bound esterase catalyzes the degradation of the polyester PUR.
ISSN:0922-338X
DOI:10.1016/S0922-338X(97)83000-0