Analysis of genetic diversity in common loon Gavia immer using RAPD and mitochondrial RFLP techniques

We used random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphisms (RFLP) of mitochondrial cytochrome b (cyt b) gene to evaluate the genetic diversity in common loon Gavia immer populations from two regions in the United States: New England (NE) and Michigan (MI). RAPD ana...

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Bibliographic Details
Published in:Molecular ecology 1997-06, Vol.6 (6), p.581-586
Main Authors: Dhar, A. K., Pokras, M. A., Garcia, D. K., Evers, D. C., Gordon, Z. J., Alcivar-Warren, A.
Format: Article
Language:English
Subjects:
cytochrome b
Freshwater
Gavia immer
genetic diversity
RAPD
RFLP
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Summary:We used random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphisms (RFLP) of mitochondrial cytochrome b (cyt b) gene to evaluate the genetic diversity in common loon Gavia immer populations from two regions in the United States: New England (NE) and Michigan (MI). RAPD analysis with 18 primers showed 74% polymorphism in NE and 50% in MI loons (similarity coefficient F= 0.92). Although no population‐specific markers were found, the frequencies of some RAPD bands varied between the two populations suggesting geographical differences. RFLP analyses with BamHI enzyme and a 307‐bp mitochondrial cyt b gene showed four haplotypes in the NE loon samples and two in the MI samples. The mtDNA haplotype diversity was 0.74 for NE and 0.51 for MI loons, supporting the RAPD data that NE loons have greater genetic diversity than MI loons.
ISSN:0962-1083
1365-294X
DOI:10.1046/j.1365-294X.1997.00218.x