Generation of mouse functional oocytes in rat by xeno-ectopic transplantation of primordial germ cells

Primordial germ cells (PGCs) are germ cell progenitors in the fetal genital ridge; female PGCs give rise to definitive oocytes that contribute to the next generation. Artificial PGCs have been induced in vitro from pluripotent stem cells and gonad-like tissue has been induced in vivo by cotransplant...

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Published in:Biology of reproduction 2014-10, Vol.91 (4), p.89-89
Main Authors: Hayama, Tomonari, Yamaguchi, Tomoyuki, Kato-Itoh, Megumi, Hamanaka, Sanae, Kawarai, Mami, Sanbo, Makoto, Tamura, Chihiro, Lee, Youn-Su, Yanagida, Ayaka, Murayama, Hideyuki, Mizuno, Naoaki, Umino, Ayumi, Sato, Hideyuki, Yamazaki, Satoshi, Masaki, Hideki, Kobayashi, Toshihiro, Hirabayashi, Masumi, Nakauchi, Hiromitsu
Format: Article
Language:English
Subjects:
Animals
Benzofurans
Embryonic Stem Cells - cytology
Embryonic Stem Cells - transplantation
Female
Germ Cells
Heterografts
Kidney - cytology
Male
Mice
Mice, Inbred ICR
Mice, SCID
Oocytes - physiology
Oogenesis - physiology
Quinolines
Rats
Rats, Inbred Strains
Stem Cell Transplantation
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Summary:Primordial germ cells (PGCs) are germ cell progenitors in the fetal genital ridge; female PGCs give rise to definitive oocytes that contribute to the next generation. Artificial PGCs have been induced in vitro from pluripotent stem cells and gonad-like tissue has been induced in vivo by cotransplantation of PGCs with PGC-free gonadal cells. To apply these technologies to human infertility treatment or conservation of rare species, PGC transplantation must be established in xenogenic animals. Here, we established a xenogeneic transplantation model by inducing ovary-like tissue from PGCs in xenogenic animals. We transplanted enzymatically dispersed PGCs with PGC-free gonadal cells under the kidney capsule of xenogenic immunodeficient animals. The transplanted cells formed ovary-like tissues under the kidney capsule. These tissues were histologically similar to the normal gonad and expressed the oocyte markers Vasa and Stella. In addition, mouse germinal vesicle-stage oocyte-like cells collected from ovary-like tissue in rats matured to metaphase II via in vitro maturation and gave rise to offspring by intracytoplasmic sperm injection. Our studies show that rat/mouse female PGCs and PGC-free gonadal cells can develop and reconstruct ovary-like tissue containing functional oocytes in an ectopic xenogenic microenvironment.
ISSN:1529-7268
DOI:10.1095/biolreprod.114.121640