Adhesion of resin-based sealers to dentine: an atomic force microscopy study

• Published in: International endodontic journal 2014-11, Vol.47 (11), p.1052-1057
• Main Authors: do Prado, M., de Assis, D. F., Gomes, B. P. F. A., Simão, R. A.
• Format: Article
• Language: English
• Subjects: adhesion; Adhesiveness; atomic force microscopy; Dentin - chemistry; Dentistry; final flush; Humans; irrigants; Microscopy, Atomic Force - methods; resin-based sealers; Resins, Synthetic - chemistry
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/iej.12247

Aim To evaluate the effect of several final irrigants on the adhesion force (Fad) between dentine and resin‐based sealers by means of atomic force microscopy (AFM). Methodology Twelve distal roots of mandibular molars were used. The roots were smoothened and cut to obtain 36 specimens. During chemomechanical preparation of the root canals, 5.25% NaOCl was used as the irrigant. The specimens were then divided into six groups according to the final irrigant used: control group – immersed in distilled water (DW) for 1 min; chlorhexidine (CHX) group – in 2% CHX for 1 min; NaOCl group – in 5.25% NaOCl for 1 min; EDTA group – in 17% EDTA for 5 min, EDTA/CHX group – in EDTA, followed by intermediate flushing with DW and then immersed in CHX; EDTA/NaOCl group – in EDTA, followed by intermediate flushing with DW and then immersed in NaOCl. After the treatments, all groups were washed with DW to remove all traces of the irrigants. Afterwards, the samples were dried and attached to a glass base. AFM tips containing AH Plus and Real Seal SE sealers were used to obtain force–distance curves with regard to dentine‐treated surfaces. Data were analysed statistically using nonparametric tests with the significance level set at P