Effect of carbon source and dissolved oxygen level on cell growth and pullulanase production by Bacillus stearothermophilus G-82

Cell growth and extracellular pullulanase production of Bacillus stearothermophilus G-82 were investigated in batch cultures using a defined medium with glucose, maltose, pullulan or amylopectin as carbon source. Maximum enzyme activity was with pullulan or amylopectin. Cell growth in batch culture...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 1992, Vol.8 (1), p.21-23
Main Authors: EMANUILOVA, E. I, KAMBOUROVA, M. S
Format: Article
Language:English
Subjects:
alpha -dextrin endo-1,6- alpha -glucosidase
amylopectin
Biological and medical sciences
Biotechnology
dissolved oxygen
Fundamental and applied biological sciences. Psychology
glucose
maltose
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
pullulan
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Summary:Cell growth and extracellular pullulanase production of Bacillus stearothermophilus G-82 were investigated in batch cultures using a defined medium with glucose, maltose, pullulan or amylopectin as carbon source. Maximum enzyme activity was with pullulan or amylopectin. Cell growth in batch culture was better under oxygen unlimited conditions, while higher total and specific enzyme activities, using pullulan or amylopectin, were obtained in oxygen-limited conditions. Enzyme accumulation took place in the late growth phase. The highest enzyme production of 300 U/l was reached when pullulan was used as carbon source in conditions of oxygen limitation.
ISSN:0959-3993
1573-0972
DOI:10.1007/BF01200678