Detection of Pediococcus spp. in brewing yeast by a rapid immunoassay

A membrane immunofluorescent-antibody, test was developed to detect diacetyl-producing Pediococcus contaminants in brewery pitching yeast (yeast [Saccharomyces cerevisiae] slurry collected for reinoculation). Centrifugations at 11 and 5,100 X g separate yeast cells from bacteria and concentrate the...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1992-02, Vol.58 (2), p.713-716
Main Authors: Whiting, M, Crichlow, M, Ingledew, W.M, Ziola, B
Format: Article
Language:English
Subjects:
Bacteria
Biological and medical sciences
brewers yeast
contamination
Fluorescent Antibody Technique
Food contamination & poisoning
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
Pediococcus
Pediococcus - growth & development
Pediococcus - isolation & purification
Saccharomyces cerevisiae
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - isolation & purification
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Summary:A membrane immunofluorescent-antibody, test was developed to detect diacetyl-producing Pediococcus contaminants in brewery pitching yeast (yeast [Saccharomyces cerevisiae] slurry collected for reinoculation). Centrifugations at 11 and 5,100 X g separate yeast cells from bacteria and concentrate the bacteria, respectively. Pelleted bacteria resuspended and trapped on a black membrane filter are reacted with monoclonal antibodies specific for cell surface antigens and then with fluorescein-conjugated indicator antibodies. Whether pitching yeast is contaminated with pediococci at 0.001% is determined in < 4 h. The sensitivity of the assay is 2 orders of magnitude below the Pediococcus detection limit of direct microscopy.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.58.2.713-716.1992