alpha-gliadin gene family. I. Characterization of ten new wheat alpha-gliadin genomic clones, evidence for limited sequence conservation of flanking DNA, and Southern analysis of the gene family

A detailed study of the organization of the wheat alpha-gliadin gene family is described. In the first stage of this study recombinant libraries of sufficient size and quality were constructed to allow examination of large, closely-related, gene families. A large number of alpha-gliadin clones were...

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Bibliographic Details
Published in:Theoretical and applied genetics 1997-07, Vol.95 (1/2), p.50-58
Main Authors: Anderson, O.D, Litts, J.C, Greene, F.C
Format: Article
Language:English
Subjects:
Biological and medical sciences
chromosome mapping
chromosomes
complementary DNA
DNA
DNA sequencing
Fundamental and applied biological sciences. Psychology
genbank/u08287
genbank/u51302
genbank/u51303
genbank/u51304
genbank/u51305
genbank/u51306
genbank/u51307
genbank/u51308
genbank/u51309
gene location
Genes. Genome
Genetic aspects
Genomics
gliadin
Molecular and cellular biology
Molecular genetics
molecular sequence data
multigene family
nucleotide sequences
Nucleotide sequencing
Physiological aspects
Plant genetics
Southern blotting
structural genes
Triticum
Triticum aestivum
Wheat
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Summary:A detailed study of the organization of the wheat alpha-gliadin gene family is described. In the first stage of this study recombinant libraries of sufficient size and quality were constructed to allow examination of large, closely-related, gene families. A large number of alpha-gliadin clones were then isolated in a partial screen of these libraries. A set of these clones were sequenced and ten new gene sequences are now reported. Including these new sequences, 20 of the 27 known alpha-gliadin genomic and cDNA sequences are from cv Cheyenne, making this cultivar the best studied for gliadin family organization. An analysis of the DNA flanking the coding regions shows divergences which may indicate the functional ends of the alpha-gliadin genes. High-resolution Southern analyses on DNA from aneuploid and chromosome-substitution lines allow most of the 20 distinct alpha-gliadin HindIII restriction fragments in cvs Cheyenne and Chinese Spring to be assigned to specific genomes. It is estimated that as many as 150 alpha-gliadin genes occur in the cultivar Cheyenne, and the limitations of such estimates are discussed.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220050531