Determination of a novel integron-located variant (blaOXA-320) of Class D β-lactamase in Proteus mirabilis

• Published in: Journal of basic microbiology 2014-10, Vol.54 (10), p.1030-1035
• Main Authors: Cicek, Aysegul Copur, Duzgun, Azer Ozad, Saral, Aysegul, Sandalli, Cemal
• Format: Article
• Language: English
• Subjects: beta-Lactamases - chemistry; beta-Lactamases - genetics; beta-Lactamases - isolation & purification; beta-Lactamases - metabolism; blaOXA-320 gene; Class D β-lactamase; Drug Resistance, Multiple, Bacterial - genetics; Humans; Integron; Integrons; Molecular Sequence Data; P. mirabilis; Proteus mirabilis - drug effects; Proteus mirabilis - genetics; Proteus mirabilis - isolation & purification; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Turkey; Urine - microbiology
• ISSN: 0233-111X; 1521-4028
• DOI: 10.1002/jobm.201300264

Proteus mirabilis (P. mirabilis) is one of Gram‐negative pathogens encountered in clinical specimens. A clinical isolate (TRP41) of P. mirabilis was isolated from a Turkish patient in Turkey. The isolate was identified using the API 32GN system and 16S rRNA gene sequencing and it was found resistant to ampicillin/sulbactam, piperacillin, tetracycline, and trimethoprim/sulfamethoxazole. This isolate was harboring a Class 1 integron gene cassette and its DNA sequence analysis revealed a novel blaOXA variant exhibiting one amino acid substitution (Asn266Ile) from blaOXA‐1. This new variant of OXA was located on Class 1 integron together with aadA1 gene encoding aminoglycoside‐modifying enzymes. According to sequence records, the new variant was named as blaOXA‐320. Cassette array and size of integron were found as blaOXA‐320‐aadA1 and 2086 bp, respectively. The blaOXA‐320 gene is not transferable according to conjugation experiment. In this study, we report the first identification of blaOXA‐320‐aadA1 gene cassette, a novel variant of Class D β‐lactamase, in P. mirabilis from Turkey.