The use of Biobrane® as a delivery method for cultured epithelial autograft in burn patients

Abstract Background Cultured epithelial autografts (CEA) are well described in the literature and are advantageous when dealing with major burns. There have been many methods of CEA application described, however they all have their own difficulties. Here we describe a novel technique of culturing t...

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Bibliographic Details
Published in:Burns 2013-08, Vol.39 (5), p.876-880
Main Authors: Frew, Q, Philp, B, Shelley, O, Myers, S, Navsaria, H, Dziewulski, P
Format: Article
Language:English
Subjects:
Adult
Biobrane
Burns
Burns - therapy
CEA
Cells, Cultured
Coated Materials, Biocompatible
Critical Care
Cultured epithelial autograft
Epithelial Cells - transplantation
Female
Humans
Infant
Keratinocytes - transplantation
Male
Skin Transplantation - methods
Tissue Engineering - methods
Transplantation, Autologous
Treatment Outcome
Young Adult
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Summary:Abstract Background Cultured epithelial autografts (CEA) are well described in the literature and are advantageous when dealing with major burns. There have been many methods of CEA application described, however they all have their own difficulties. Here we describe a novel technique of culturing the keratinocytes in Biobrane®. Methods Skin samples were taken from three patients and cultured into pre-confluent keratinocytes. These were seeded in Biobrane® and applied directly to the patients’ wounds. Results Three patients had Biobrane® with seeded keratinocytes applied. The Biobrane was applied to both donor and burn wound sites, with healing times being similar to the keratinocyte sheets. Conclusion The experience of the authors shows that using Biobrane® seeded with keratinocytes was easier to handle and quicker to produce than confluent sheets of keratinocytes, with no perceived disadvantages to the patients.
ISSN:0305-4179
1879-1409
DOI:10.1016/j.burns.2012.11.013