Mutational modifications of hepatitis A virus proteins 2B and 2C described for cell culture-adapted and attenuated virus are present in wild-type virus populations

Studies have identified certain mutations in the 2B and 2C proteins of hepatitis A virus (HAV) as being essential for efficient growth in cultured cells, and it is assumed that these mutations contribute to the attenuated phenotype. We found that mutations supporting cell culture growth already exis...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2014-10, Vol.159 (10), p.2699-2704
Main Authors: Weilandt, Rebecca, Paulmann, Dajana, Schlottau, Kore, Vallbracht, Angelika, Dotzauer, Andreas
Format: Article
Language:English
Subjects:
Adaptation
Adaptation, Physiological - genetics
Amino acids
Base Sequence
Biomedical and Life Sciences
Biomedicine
Brief Report
Carrier Proteins - genetics
Cell culture
Cell Line
Child, Preschool
Cloning
cultured cells
Feces - virology
Hepatitis A
Hepatitis A virus
Hepatitis A virus - genetics
Hepatitis A virus - isolation & purification
Hepatitis A Virus, Human
Humans
Infections
Infectious Diseases
Kinases
Male
Medical Microbiology
Mutation
phenotype
Proteins
RNA, Viral - analysis
Sequence Analysis, RNA
Viral Nonstructural Proteins - genetics
Virology
Virus Replication - genetics
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Studies have identified certain mutations in the 2B and 2C proteins of hepatitis A virus (HAV) as being essential for efficient growth in cultured cells, and it is assumed that these mutations contribute to the attenuated phenotype. We found that mutations supporting cell culture growth already exist in wild-type HAV populations. This suggests that these variants are not entirely generated de novo but are selected from the wild-type population. In a prolonged case of hepatitis A, we found that sequences associated with cell culture adaptation predominated later in infection. This might suggest selection of an attenuated virus population during a prolonged clinical infection.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-014-2103-6