Characterization of the m4 muscarinic receptor Ca super(2+) response in a subclone of PC-12 cells by single cell flow cytometry. Inhibition of the response by bradykinin

We have studied Ca super(2+) mobilization mediated by the constitutively expressed muscarinic receptor on a subclone of PC-12 cells. The subclone, ACH2, was isolated with a flow cytometer by selection of single cells that exhibited a strong intracellular Ca super(2+) response to acetylcholine (ACh)....

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Bibliographic Details
Published in:The Journal of biological chemistry 1991-01, Vol.266 (18), p.11738-11745
Main Authors: Ransom, J T, Cherwinski, H M, Delmendo, R E, Sharif, NA, Eglen, R
Format: Article
Language:English
Subjects:
bradykinin
calcium
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Summary:We have studied Ca super(2+) mobilization mediated by the constitutively expressed muscarinic receptor on a subclone of PC-12 cells. The subclone, ACH2, was isolated with a flow cytometer by selection of single cells that exhibited a strong intracellular Ca super(2+) response to acetylcholine (ACh). Cell to cell heterogeneity of resting Ca super(2+) levels was markedly reduced in the subclone and homogeneity of the population response was also dramatically. The signaling mechanism coupled to the m4 muscarinic receptor can be inhibited by signals initiated by the bradykinin receptor.
ISSN:0021-9258