Flow cytometric evaluation of the effects of doxorubicin on rat spermatogenesis

Histopathologic examination of testicular tissue allows testicular impairment to be investigated. As an alternative to histopathology, flow cytometry (FCM) using a triple staining technique that combines DNA-ploidy with mitochondria stainablitiy and vimentin immunostaining has also been utilized to...

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Bibliographic Details
Published in:Reproductive toxicology (Elmsford, N.Y.) N.Y.), 1997-07, Vol.11 (4), p.521-531
Main Authors: Suter, L, Bobadilla, M, Koch, E, Bechter, R
Format: Article
Language:English
Subjects:
Animals
Antibiotics, Antineoplastic - toxicity
Biological and medical sciences
Body Weight - drug effects
doxorubicin
Doxorubicin - toxicity
Drug toxicity and drugs side effects treatment
Flow Cytometry
histopathology
Male
Medical sciences
Organ Size - drug effects
Pharmacology. Drug treatments
rat
Rats
Rats, Wistar
Sperm Count - drug effects
sperm head counts
spermatogenesis
Spermatogenesis - drug effects
Testis - drug effects
time course
Toxicity: urogenital system
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Summary:Histopathologic examination of testicular tissue allows testicular impairment to be investigated. As an alternative to histopathology, flow cytometry (FCM) using a triple staining technique that combines DNA-ploidy with mitochondria stainablitiy and vimentin immunostaining has also been utilized to evaluate testicular damage. In this article we evaluate the effects on spermatogenesis after acute exposure of rats to doxorubicin. Testicular cell suspensions of treated and control animals were analyzed by FCM. This allows several cell types to be identified and quantified, giving a control pattern. Deviations from this control pattern are considered as an indication of testicular damage. Doxorubicin produced a depletion of spermatogonia as early as 3 d after treatment. This effect could be followed through the temporal evolution of spermatogenesis. Comparable results were obtained by histopathology. The presented results show that FCM is a suitable and sensitive method for the detection of testicular damage. The advantages of FCM over other techniques include its rapidity and objectivity.
ISSN:0890-6238
1873-1708
DOI:10.1016/S0890-6238(97)00019-1