Development of slide ELISA (SELISA) for detection of four poultry viral pathogens by direct heat fixation of viruses on glass slides

•To detect poultry viral agents by SELISA.•The positive signals for viral aggregates were detected under light microscope. The development of an easy and simpler method of slide enzyme-linked immunosorbent assay (SELISA) for the diagnosis of four economically important poultry viruses viz., Newcastl...

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Bibliographic Details
Published in:Journal of virological methods 2014-12, Vol.209, p.76-81
Main Authors: Desingu, P.A., Singh, S.D., Dhama, K., Vinodh Kumar, O.R., Singh, R., Singh, R.K.
Format: Article
Language:English
Subjects:
Animals
DNA Viruses - isolation & purification
Enzyme-Linked Immunosorbent Assay - methods
Glass
Hot Temperature
Microscopy
Poultry
Poultry Diseases - diagnosis
Poultry Diseases - virology
Poultry viruses
RNA Viruses - isolation & purification
Semi quantitation
Slide ELISA
Specimen Handling - methods
Veterinary Medicine - methods
Virology - methods
Virus Diseases - diagnosis
Virus Diseases - veterinary
Virus Diseases - virology
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Summary:•To detect poultry viral agents by SELISA.•The positive signals for viral aggregates were detected under light microscope. The development of an easy and simpler method of slide enzyme-linked immunosorbent assay (SELISA) for the diagnosis of four economically important poultry viruses viz., Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious bursal disease virus (IBDV) and egg drop syndrome 76 virus (EDS 76) and the use of SELISA for semi quantitation of NDV are described. The positive signals for viral aggregates were detected under light microscope. This is the first report regarding the development of SELISA based on heat fixation for the diagnosis of viral pathogens.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2014.08.010