Glucose sensitivity of polypyrrole films containing immobilized glucose oxidase and hydroquinonesulfonate ions

Glucose oxidase (GOx) and hydroquinonesulfonate ions (HQS) were incorporated into polypyrrole by electropolymerization of pyrrole in the presence of these substances. The resulting polymer films showed sensitivities to glucose when no mediator was used in solution, the magnitude of the sensitivity b...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1991-01, Vol.63 (1), p.49-54
Main Authors: Kajiya, Yoshio, Sugai, Hiroko, Iwakura, Chiaki, Yoneyama, Hiroshi
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
glucose
hydroquinonesulfonate
Immobilization of organelles and whole cells
Immobilization techniques
Methods. Procedures. Technologies
polypyrrole films
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Summary:Glucose oxidase (GOx) and hydroquinonesulfonate ions (HQS) were incorporated into polypyrrole by electropolymerization of pyrrole in the presence of these substances. The resulting polymer films showed sensitivities to glucose when no mediator was used in solution, the magnitude of the sensitivity being influenced by the concentration of HQS at a fixed concentration of GOx. The amount of immobilized HQS and GOx was proportional to the deposition charge of the polypyrrole films, but there was an optimum deposition charge to achieve the highest sensitivity. Absorption spectra and fluorescence spectra measurements of HQS and GOx in the deposition bath suggested that HQS adsorbs on GOx in such a way as to allow electronic interaction between each other.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac00001a009