A chloramphenicol‐streptomycin‐resistance plasmid from a clinical strain of Staphylococcus sciuri and its structural relationships to other staphylococcal resistance plasmids

: A 5.1‐kb plasmid, designated pSCS12, isolated from a naturally occurring Staphylococcus sciuri conferred resistance to chloramphenicol (CmR) and streptomycin (SmR). Restriction endonuclease analyses of pSCS12 revealed partial structural homologies to the CmR‐plasmids pC221 from S. aureus and pSCS1...

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Bibliographic Details
Published in:FEMS microbiology letters 1991-08, Vol.82 (3), p.319-322
Main Authors: Schwarz, Stefan, Grölz‐Krug, Sabine
Format: Article
Language:English
Subjects:
Antibiotic resistance
Bacteriology
Biological and medical sciences
Chloramphenicol
Chloramphenicol Resistance - genetics
Drug Resistance, Microbial - genetics
Fundamental and applied biological sciences. Psychology
Genetics
Microbiology
Plasmid
R Factors - genetics
Restriction Mapping
Staphylococcus - drug effects
Staphylococcus - genetics
Staphylococcus sciuri
Streptomycin
Streptomycin - pharmacology
Transfection
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Summary:: A 5.1‐kb plasmid, designated pSCS12, isolated from a naturally occurring Staphylococcus sciuri conferred resistance to chloramphenicol (CmR) and streptomycin (SmR). Restriction endonuclease analyses of pSCS12 revealed partial structural homologies to the CmR‐plasmids pC221 from S. aureus and pSCS1 from S. intermedius, to the SmR‐plasmids pSAI‐1 from S. hyicus and pS194 from S. aureus, as well as to the CmR/SmR plasmid pSK68 from S. aureus. Southern‐blot hybridization with specific CmR‐ and SmR‐gene probes confirmed these similarities and allowed the mapping of the CmR‐ and SmR‐determinants in the S. sciuri plasmid pSCS12. These observations lead to the suggestion that CmR/SmR‐plasmids, such as pSCS12, may have evolved from CmR‐ and SmR‐plasmids by interplasmidic recombination.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1991.tb04902.x