Catalytic Subunits of Aplysia Neuronal cAMP-Dependent Protein Kinase with Two Different N Termini

Previously, two forms of cAMP-dependent protein kinase catalytic subunit generated by mutually exclusive use of two internal exon cassettes (A1 and A2) were demonstrated in Aplysia neurons. Here, it is shown that there also exist catalytic subunits with alternative N termini derived from two exons,...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1992-03, Vol.89 (5), p.1641-1645
Main Authors: Beushausen, Sven, Lee, Eric, Walker, Barbara, Bayley, Hagan
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
AMP
Analytical, structural and metabolic biochemistry
Animals
Antibodies
Aplysia
Aplysia - enzymology
Aplysia - genetics
Base Sequence
Biological and medical sciences
Biotechnology
Blotting, Western
Complementary DNA
control
cyclic AMP
enzymatic activity
Enzymes and enzyme inhibitors
Exons
Fundamental and applied biological sciences. Psychology
Genes
Marine
marine molluscs
Medical research
Molecular Sequence Data
Nervous system
Neurons
Promoter regions
Promoter Regions, Genetic
Protein Kinases - chemistry
Protein Kinases - genetics
Protein Kinases - immunology
Proteins
Restriction Mapping
RNA probes
RNA Splicing
RNA, Messenger - genetics
Transferases
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Summary:Previously, two forms of cAMP-dependent protein kinase catalytic subunit generated by mutually exclusive use of two internal exon cassettes (A1 and A2) were demonstrated in Aplysia neurons. Here, it is shown that there also exist catalytic subunits with alternative N termini derived from two exons, N1 and N2, expressed in combination with either of the internal cassettes. Processed transcripts including N1 or N2 sequences are of about equal abundance in the nervous system, arise through alternative promoter use, and encode catalytically active polypeptides. The N2 amino acid sequence is 21 residues longer than the N1 sequence and is homologous to the nonmyristoylated N terminus of the TPK1 gene product, a yeast catalytic subunit homolog. These data support the view that cAMP-dependent protein kinase activity in Aplysia neurons is produced by a complex array of regulatory and catalytic subunits that generate multiple holoenzymes with a spectrum of properties.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.89.5.1641