Expression and prognostic value of Ars2 in hepatocellular carcinoma

Background Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in China. Arsenic resistance protein 2 (Asr2) was reported to be important for microRNA (miR) biogenesis, and its depletion could reduce the levels of several miRs, including miR-21, which is over-expressed in HCC....

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Bibliographic Details
Published in:International journal of clinical oncology 2014-10, Vol.19 (5), p.880-888
Main Authors: He, Qian, Huang, Yongde, Cai, Lei, Zhang, Shaobo, Zhang, Chenghua
Format: Article
Language:English
Subjects:
Adult
Aged
Apoptosis - genetics
Cancer Research
Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - pathology
Cell Proliferation
China
Female
Gene Expression Regulation, Neoplastic
Humans
Liver cancer
Liver Neoplasms - genetics
Liver Neoplasms - pathology
Male
Medical prognosis
Medicine
Medicine & Public Health
MicroRNAs - biosynthesis
Middle Aged
Nuclear Proteins - biosynthesis
Nuclear Proteins - genetics
Oncology
Original Article
Prognosis
Protein expression
Surgical Oncology
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Summary:Background Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in China. Arsenic resistance protein 2 (Asr2) was reported to be important for microRNA (miR) biogenesis, and its depletion could reduce the levels of several miRs, including miR-21, which is over-expressed in HCC. We hypothesized that Ars2 is also overexpressed in HCC and may be involved in the biological properties of HCC. Methods Ars2 immunolabeling was evaluated in 132 HCCs. Ars2 immunolabeling, Ars2 qRT-PCR and miR-21 were evaluated in 20 HCCs and in paired normal tissues. Ars2 shRNA was transfected into SMCC-7721 and HepG2 HCC cells. The cell proliferation and expression of Ars2 and miR-21 were subsequently evaluated. Results Ars2 was expressed primarily in the nucleus of HCC cells. The expression of Ars2 was statistically correlated with the loss of HCC differentiation and pathological stage. The survival rates of patients with low Ars2 expression in HCC were statistically higher than patients with overexpressed Ars2 in HCC. Ars2 and miR-21 were more highly expressed in HCC specimens than normal tissues, and they were also correlated. The knockdown of Ars2 in HCC cells inhibited miR-21 expression and cell proliferation. Conclusions Ars2 is overexpressed in HCC and may have prognostic value; it might play an important role in HCC proliferation and miR-21 expression.
ISSN:1341-9625
1437-7772
DOI:10.1007/s10147-013-0642-6