Isolation and characterization of microsatellite loci for the blackstripe topminnow Fundulus notatus and their variability in two closely related species

A total of 17 polymorphic microsatellite loci were isolated from the blackstripe topminnow Fundulus notatus. In a sample of 29 individuals, these loci were found to possess two to 19 alleles with expected heterozygosity values ranging from 0·212 to 0·919 and all but one of the loci conformed to Hard...

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Bibliographic Details
Published in:Journal of fish biology 2014-11, Vol.85 (5), p.1726-1732
Main Authors: Feldheim, K. A., Kreiser, B. R., Schmidt, B., Duvernell, D. D., Schaefer, J. F.
Format: Article
Language:English
Subjects:
Alleles
Animals
cross-species amplification
Cyprinidae - genetics
Fundulidae
Genetic Loci
genetic marker
Microsatellite Repeats
population genetics
primer sequence
Sequence Analysis, DNA
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Summary:A total of 17 polymorphic microsatellite loci were isolated from the blackstripe topminnow Fundulus notatus. In a sample of 29 individuals, these loci were found to possess two to 19 alleles with expected heterozygosity values ranging from 0·212 to 0·919 and all but one of the loci conformed to Hardy–Weinberg equilibrium expectations. Many of these loci were polymorphic in the closely related species Fundulus olivaceus and Fundulus euryzonus providing a set of markers that should prove useful in future ecological and evolutionary studies of members of this species complex.
ISSN:0022-1112
1095-8649
DOI:10.1111/jfb.12469