Isolation and characterization of siderophore from cowpea Rhizobium (peanut isolate)

In an iron-depleted broth culture of cowpea Rhizobium (a peanut isolate), phenolate type of compounds were detected. Chemical characterization showed the presence of 2,3-dihydroxy benzoic acid (DHBA) and 3,4-DHBA in the siderophore extract. Lysine and alanine were identified as conjugated amino acid...

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Bibliographic Details
Published in:Current microbiology 1992-03, Vol.24 (3), p.137-141
Main Authors: Jadhav, R.S. (M.S. University of Baroda, Baroda, Gujarat, India), Desai, A.J
Format: Article
Language:English
Subjects:
ACIDE PHENOLIQUE
ACIDOS FENOLICOS
ALANINA
ALANINE
Arachis hypogaea
Bacteriology
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
CHELATEUR
CRECIMIENTO
CROISSANCE
FER
Fundamental and applied biological sciences. Psychology
GLICINA
GLYCINE (ACIDE AMINE)
HIERRO
LISINA
LYSINE
Microbiology
Permeability, membrane transport, intracellular transport
QUELATOS
RHIZOBIUM
SIDEROFOROS
SIDEROPHORE
SUBSTRAT DE CULTURE
SUSTRATO DE CULTIVO
THREONINE
TRANSPORT DES SUBSTANCES NUTRITIVES
TRANSPORTE DE NUTRIENTES
TREONINA
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Summary:In an iron-depleted broth culture of cowpea Rhizobium (a peanut isolate), phenolate type of compounds were detected. Chemical characterization showed the presence of 2,3-dihydroxy benzoic acid (DHBA) and 3,4-DHBA in the siderophore extract. Lysine and alanine were identified as conjugated amino acids of the siderophore. Maximum concentration of the siderophore in the culture supernatant was found after 24 h of growth. The compounds in the extracted siderophore induced growth of Rhizobium in a medium containing EDTA. Addition of lysine and alanine in the growth medium (20 mM each) led to a fourfold increase in siderophore production
ISSN:0343-8651
1432-0991
DOI:10.1007/BF01568978