A novel cost-effective assay based on real-time PCR for COMT Val158Met genotyping

Abstract A functional polymorphism in the catechol-O-methyltransferase (COMT) gene (Val158Met) has been associated with a large number of human diseases and endophenotypes. The aim of this study was to develop a novel cost-effective assay to genotype this polymorphism. The novel assay was based on t...

Full description

Saved in:
Bibliographic Details
Published in:Biomarkers 2014-11, Vol.19 (7), p.567-570
Main Authors: Ojeda, Diego A., López-León, Sandra, Forero, Diego A.
Format: Article
Language:English
Subjects:
Catechol O-Methyltransferase - genetics
Cost-Benefit Analysis
Genetic Testing - economics
Genetic Testing - methods
Genotype
Health Care Costs
Humans
Phenotype
Polymorphism, Single Nucleotide
Predictive Value of Tests
Real-Time Polymerase Chain Reaction - economics
Reproducibility of Results
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract A functional polymorphism in the catechol-O-methyltransferase (COMT) gene (Val158Met) has been associated with a large number of human diseases and endophenotypes. The aim of this study was to develop a novel cost-effective assay to genotype this polymorphism. The novel assay was based on the combination of allele-specific PCR and high-resolution melting in a qPCR platform. Melt-curve analysis allowed a clear differentiation of the three genotypes. This novel assay could be implemented in the study of a large number of diseases and endophenotypes related to COMT dysfunction and could be extended for the analysis of other functional polymorphisms.
ISSN:1354-750X
1366-5804
DOI:10.3109/1354750X.2014.948069