Correlation between spermatogenesis disorders and rat testes CYP2E1 mRNA contents under experimental alcoholism or type I diabetes

• Published in: Advances in medical sciences 2014-09, Vol.59 (2), p.183-189
• Main Authors: Shayakhmetova, Ganna M., Bondarenko, Larysa B., Matvienko, Anatoliy V., Kovalenko, Valentina M.
• Format: Article
• Language: English
• Subjects: Alcoholism; Alcoholism - physiopathology; Animals; Correlation; CYP2E1 expression; Cytochrome P-450 CYP2E1 - genetics; Cytochrome P-450 CYP2E1 - metabolism; Diabetes; Diabetes Mellitus, Type 1 - complications; Disease Models, Animal; Enzyme Induction; Infertility, Male - complications; Infertility, Male - etiology; Infertility, Male - metabolism; Infertility, Male - pathology; Male; Rats; Rats, Wistar; RNA, Messenger - metabolism; Spermatogenesis; Testis - metabolism; Testis - pathology
• ISSN: 1896-1126; 1898-4002
• DOI: 10.1016/j.advms.2014.03.004

The aim of the study was to investigate the correlation between spermatogenesis disorders and CYP2E1 mRNA contents in testes of rats with experimental alcoholism or type I diabetes. Two pathological states characterized by CYP2E1 induction were simulated on Wistar male rats: experimental alcoholism and type I diabetes. As controls for each state, equal number of animals (of the same age and weight) were used. Morphological evaluation of rat testes was carried out. The spermatogenic epithelium state was estimated by four points system. CYP2E1 mRNA expression was rated by method of reverse transcriptase polymerase chain reaction. Pearson correlation coefficients were used for describing relationships between variables. The presence of alcoholism and diabetes-mediated quantitative and qualitative changes in male rat spermatogenic epithelium in comparison with norm has been demonstrated. The increased levels of testes CYP2E1 have been fixed simultaneously. CYP2E1 mRNA content negatively strongly correlated with spermatogenic index value (r=−0.99; P