Dried umbilical cord is a potential material for retrospective diagnosis of intrauterine enterovirus infection

Abstract Objective: To determine whether dried umbilical cords (UCs) are useful for retrospective diagnosis of intrauterine enterovirus (EV) infection. Methods: Dried UCs in two patients with neonatal EV sepsis and 10 neonates without infectious signs were enrolled. Viral RNA was extracted from thei...

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Bibliographic Details
Published in:The journal of maternal-fetal & neonatal medicine 2014-11, Vol.27 (17), p.1820-1822
Main Authors: Morioka, Ichiro, Matsumoto, Masaaki, Miwa, Akihiro, Yokota, Tomoyuki, Matsuo, Kiyomi, Koda, Tsubasa, Nagasaka, Miwako, Shibata, Akio, Fujita, Kaori, Yamane, Masayuki, Yamada, Hideto, Enomoto, Miki, Chikahira, Masatsugu, Iijima, Kazumoto
Format: Article
Language:English
Subjects:
Asymptomatic Infections - epidemiology
Coxsackievirus
Desiccation
echovirus
Enterovirus Infections - diagnosis
Enterovirus Infections - transmission
Female
Humans
Infant, Newborn
Infant, Newborn, Diseases - diagnosis
Infant, Newborn, Diseases - virology
Infectious Disease Transmission, Vertical
Male
Persistent Fetal Circulation Syndrome - diagnosis
Persistent Fetal Circulation Syndrome - virology
persistent pulmonary hypertension of the newborn
Pregnancy
Pregnancy Complications, Infectious - diagnosis
Pregnancy Complications, Infectious - virology
Retrospective Studies
RNA, Viral - analysis
sepsis
Tachycardia - congenital
Tachycardia - diagnosis
Tachycardia - virology
transmission route
Umbilical Cord - chemistry
Umbilical Cord - virology
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Summary:Abstract Objective: To determine whether dried umbilical cords (UCs) are useful for retrospective diagnosis of intrauterine enterovirus (EV) infection. Methods: Dried UCs in two patients with neonatal EV sepsis and 10 neonates without infectious signs were enrolled. Viral RNA was extracted from their dried UCs, and nested reverse transcription polymerase chain reaction (RT-PCR) was performed. Results: Infection routes estimated by the clinical course were intrauterine infection in Case 1 and post-natal horizontal infection in Case 2. EV-RNA was detected from dried UC in Case 1, but not in Case 2 and 10 neonates. Conclusions: This report showed the potential use of dried UCs for retrospective diagnosis of intrauterine EV infection.
ISSN:1476-7058
1476-4954
DOI:10.3109/14767058.2013.879701