Xanthine Derivatives Quantification in Serum by Capillary Zone Electrophoresis

A capillary electrophoresis method was developed to quantify caffeine and theophylline, xanthine derivatives with bronchodilator activity. Buffer concentration, pH and applied voltage were optimized using a central composite design-face centred. Separation conditions were: silica capillary tube, 75...

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Bibliographic Details
Published in:Journal of chromatographic science 2014-10, Vol.52 (9), p.1121-1126
Main Authors: Peris-Vicente, Juan, Rambla-Alegre, Maria, Durgavanshi, Abhilasha, Bose, Devasish, Esteve-Romero, Josep, Marco-Peiró, Sergio
Format: Article
Language:English
Subjects:
Buffers
Caffeine - blood
Electrophoresis, Capillary - instrumentation
Electrophoresis, Capillary - methods
Hydrogen-Ion Concentration
Limit of Detection
Reproducibility of Results
Sensitivity and Specificity
Theophylline - blood
Xanthines - blood
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Summary:A capillary electrophoresis method was developed to quantify caffeine and theophylline, xanthine derivatives with bronchodilator activity. Buffer concentration, pH and applied voltage were optimized using a central composite design-face centred. Separation conditions were: silica capillary tube, 75 μm (i.d.) and 61 cm (total length); absorbance detection, 280 nm; borate buffer, 20 mM, pH 9.0; applied voltage, 25 kV and 1 psi injection/8 s. Validation was performed in blank serum following the International Conference Harmonization guidelines: resolution (peaks without overlapping), linear range (0.125–50 µg/mL; r 2 > 0.9999), limits of detection and quantification (10; 20 and 33; 66 ppb for caffeine and theophylline, respectively), intra- and inter-day precision (Relative standard deviation lower than 1.9%) and accuracy (98–101%). Migration times were
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/bmt164