Increased yield of fructose from glucose employing thermophilic xylose isomerase in water-ethanol mixtures

The isomerization of D-glucose in mixed ethanol-water was studied at various reaction temperatures (40-70 degrees C), employing glucose isomerase from Streptomyces phaeochromogenes and Clostridium thermohydrosulfuricum, respectively. The thermophilic Clostridium enzyme was considerably more stable t...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 1991-11, Vol.13 (11), p.787-792
Main Authors: Nilsson, K.G.I, Mosbach, K, Sakaguchi, K
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Clostridium
Clostridium thermohydrosulfuricum
enzyme activity
ethanol
fructose
fructose syrup
Fundamental and applied biological sciences. Psychology
glucose
glucose isomerase
glucose-6-phosphate isomerase
isomerases
isomerization
Microbiology
mixtures
Streptomyces
Streptomyces phaeochromogenes
temperature
Thermoanaerobacter thermohydrosulfuricus
water
xylose
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The isomerization of D-glucose in mixed ethanol-water was studied at various reaction temperatures (40-70 degrees C), employing glucose isomerase from Streptomyces phaeochromogenes and Clostridium thermohydrosulfuricum, respectively. The thermophilic Clostridium enzyme was considerably more stable towards the combination of organic cosolvent and increased temperature and with this enzyme a 55% yield of fructose from glucose was obtained at relatively low concentration of ethanol (40%).
ISSN:0141-5492
1573-6776
DOI:10.1007/bf01026760