Endocrine disruption in brook trout ( Salvelinus fontinalis) exposed to leachate from a public refuse dump

• Published in: The Science of the total environment 2003-04, Vol.305 (1), p.87-103
• Main Authors: Noaksson, Erik, Linderoth, Maria, Bosveld, Albertus T.C., Norrgren, Leif, Zebühr, Yngve, Balk, Lennart
• Format: Article
• Language: English
• Subjects: 17 β-oestradiol (E2); Agnatha. Pisces; Animal, plant and microbial ecology; Animals; Applied ecology; Aromatase (P450arom); Biological and medical sciences; Brook trout ( Salvelinus fontinalis); Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on vertebrates; Endocrine disruption; endocrine disruptors; Endocrine System - drug effects; Female; Field study; Fundamental and applied biological sciences. Psychology; Geologic Sediments; Gonads - drug effects; Gonads - growth & development; Male; Perca fluviatilis; Perch ( Perca fluviatilis); Public refuse dump; Refuse Disposal; Reproductive disorder; Salvelinus fontinalis; Sexual Maturation - drug effects; Skin Diseases - etiology; Skin Diseases - veterinary; Testosterone (T); Trout - physiology; Water Pollutants, Chemical - adverse effects
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/S0048-9697(02)00503-X

Lake Molnbyggen was previously found to harbour a large number of sexually immature female perch ( Perca fluviatilis) suffering from endocrine disruption. In an attempt to pin-point the source of the endocrine-disrupting substance(s) (EDSs), brook trout ( Salvelinus fontinalis) from Vadbäcken, a stream contaminated by leachate from a public refuse dump and which empties into Lake Molnbyggen, were investigated. In addition, female perch from Lakes Yxen and Kvarntjärn, located up-stream and down-stream of Lake Molnbyggen, were investigated. Only 16.7% of the adult female brook trout in Vadbäcken were sexually mature, associated with decreased gonadosomatic index, lower brain aromatase activity, and lower circulating levels of testosterone and 17 β-oestradiol, in comparison to female brook trout from the reference stream Björntjärnsbäcken. Male brook trout showed decreased gonadosomatic index, in addition to bile duct hyperplasia in the liver, which was also found in female brook trout livers from Vadbäcken. In Lake Molnbyggen, 57.6% of the female perch were found to be sexually immature with high frequencies of skin lesions, such as sores and fin erosion, significantly decreased gonadosomatic index, lower aromatase activity, and lower levels of testosterone and 17 β-oestradiol. No signs of reproductive disorders or endocrine disruption were seen in female perch from Lakes Yxen and Kvarntjärn compared to female perch from the reference lake, Lake Djursjön. Since brook trout of both sexes from Vadbäcken displayed the same kind of serious adverse impairment of gonad development and endocrine disruption as perch from Lake Molnbyggen, very strong evidence are provided that the refuse dump is the source for the responsible EDS(s), since both Vadbäcken and Lake Molnbyggen are known to be contaminated by leachate from that dump. The low levels of PAHs and PCBs in the surface sediments of Lake Molnbyggen suggest that these pollutants are not the responsible EDS(s).