Chemical characterization of the O-specific chain of Sphaerotilus natans ATCC 13338 lipopolysaccharide

The chemical structure of the O-specific chain of the Sphaerotilus natans lipopolysaccharide was established. The isolated polysaccharide moiety contained neutral sugars (rhamnose, glucose, and L-glycero-D-mannoheptose), 3-deoxy-D-manno-octulosonic acid (Kdo), phosphate and ethanolamine. Alditol ace...

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Bibliographic Details
Published in:Archives of microbiology 1991-08, Vol.156 (3), p.176-180
Main Authors: Masoud, Hussein, Neszmelyi, Andràs, Mayer, Hubert
Format: Article
Language:English
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Summary:The chemical structure of the O-specific chain of the Sphaerotilus natans lipopolysaccharide was established. The isolated polysaccharide moiety contained neutral sugars (rhamnose, glucose, and L-glycero-D-mannoheptose), 3-deoxy-D-manno-octulosonic acid (Kdo), phosphate and ethanolamine. Alditol acetate and methylation analyses showed that the O-specific chain contained linear pentasaccharide repeating units, composed of four units of rhamnose, substituted at 3-position, and one unit of glucose, substituted at 4-position. Oxidation by chromium trioxide showed that all sugars were alpha -linked. The structure proposed for the O-specific chain has been confirmed by periodate oxidation, and by super(H)- and super(13)C-NMR spectroscopic studies for the original and the Smith-degraded PS moiety.
ISSN:0302-8933
1432-072X
DOI:10.1007/BF00249111