Fate of Salmonella enterica serovar Typhimurium on carrots and radishes grown in fields treated with contaminated manure composts or irrigation water

• Published in: Applied and Environmental Microbiology 2004-04, Vol.70 (4), p.2497-2502
• Main Authors: Islam, M, Morgan, J, Doyle, M.P, Phatak, S.C, Millner, P, Jiang, X
• Format: Article
• Language: English
• Subjects: agricultural soils; Agriculture; alkalinity; Animals; bacterial contamination; Biological and medical sciences; carrots; Cattle; cattle manure; composted manure; Composting; crop production; Daucus carota; Daucus carota - microbiology; food contamination; Food Microbiology; food pathogens; Freshwater; Fundamental and applied biological sciences. Psychology; irrigation water; Manure - microbiology; manure spreading; Microbiology; pathogen survival; Poultry; poultry manure; radishes; Raphanus - microbiology; Raphanus sativus; Salmonella; Salmonella enterica; Salmonella enterica subsp. enterica serovar Typhimurium; Salmonella typhimurium - growth & development; Salmonella typhimurium - isolation & purification; soil amendments; Soil Microbiology; soil pollution; Soil testing; Vegetables; Water Microbiology; water pollution
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.70.4.2497-2502.2004

Three different types of compost, PM-5 (poultry manure compost), 338 (dairy cattle manure compost), and NVIRO-4 (alkaline-pH-stabilized dairy cattle manure compost), and irrigation water were inoculated with an avirulent strain of Salmonella enterica serovar Typhimurium at 10(7) CFU g-1 and 10(5) CFU ml-1, respectively, to determine the persistence of salmonellae in soils containing these composts, in irrigation water, and also on carrots and radishes grown in these contaminated soils. A split-plot block design plan was used for each crop, with five treatments (one without compost, three with each of the three composts, and one without compost but with contaminated water applied) and five replicates for a total of 25 plots for each crop, with each plot measuring 1.8 x 4.6 m. Salmonellae persisted for an extended period of time, with the bacteria surviving in soil samples for 203 to 231 days, and were detected after seeds were sown for 84 and 203 days on radishes and carrots, respectively. Salmonella survival was greatest in soil amended with poultry compost and least in soil containing alkaline-pH-stabilized dairy cattle manure compost. Survival profiles of Salmonella on vegetables and soil samples contaminated by irrigation water were similar to those observed when contamination occurred through compost. Hence, both contaminated manure compost and irrigation water can play an important role in contaminating soil and root vegetables with salmonellae for several months.