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Protective effect of CDP-choline on ischemia-reperfusion-induced myocardial tissue injury in rats
Background CDP-choline exerts tissue protective effect in several ischemic conditions. Recently we have reported that the drug prevents cardiac arrhythmias and improves survival rate in short-term myocardial ischemia reperfusion in rats. Aim In the current study, we determined the effect of intraven...
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| Published in: | Irish journal of medical science 2014-12, Vol.183 (4), p.539-548 |
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| cites | cdi_FETCH-LOGICAL-c414t-23801e4d29874748918f1e647d62000842e10ef25a426e15f955026191c8cd7a3 |
| container_end_page | 548 |
| container_issue | 4 |
| container_start_page | 539 |
| container_title | Irish journal of medical science |
| container_volume | 183 |
| creator | Coskun, C. Avci, B. Yalcin, M. Yermezler, A. Yilmaz, M. S. Savci, V. |
| description | Background
CDP-choline exerts tissue protective effect in several ischemic conditions. Recently we have reported that the drug prevents cardiac arrhythmias and improves survival rate in short-term myocardial ischemia reperfusion in rats.
Aim
In the current study, we determined the effect of intravenously administered CDP-choline on myocardial tissue injury induced by 30-min ischemia followed by 3-h reperfusion in anesthetized rats.
Methods
Myocardial ischemia was produced by ligature of the left main coronary artery. CDP-choline (100–500 mg/kg) was intravenously injected in the middle of the ischemic period. Cardiovascular parameters were recorded through the experimental period. At the end of the reperfusion period, the hearts of the animals were removed and stained for the investigation of tissue necrosis and apoptosis. The infarct size was evaluated as the ratio of the infarct area to the risk area. Apoptotic activation was assessed by TUNEL assay. Also the blood samples of rats were collected for the measurement of M30–M65, ADMA, homocysteine, and lactate levels.
Results
Ischemia/reperfusion caused serious injury in myocardium, increased blood ADMA and lactate levels without influencing other parameters. CDP-choline significantly reduced the infarct size and the number of apoptotic cells in the risk area. Blood pressure increased after CDP-choline injection; however, it returned back to the basal levels before the onset of reperfusion. CDP-choline failed to alter any other measured parameters.
Conclusion
The present results demonstrate that intravenously administered CDP-choline is able to protect myocardium from injury induced by long-term coronary occlusion–reperfusion in rats. The inhibition of apoptosis by the drug may contribute to its protective effect. But neither the increase in blood pressure in response to CDP-choline injection nor changes in plasma ADMA concentration appear to mediate the attenuation of the myocardial injury. |
| doi_str_mv | 10.1007/s11845-013-1046-3 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1618139254</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1618139254</sourcerecordid><originalsourceid>FETCH-LOGICAL-c414t-23801e4d29874748918f1e647d62000842e10ef25a426e15f955026191c8cd7a3</originalsourceid><addsrcrecordid>eNp9kEFP3DAQhS3UCrbAD-BS-cjFxWM7jnNESwtISOUAZ8s4Y_Aqibd2grT_HqMFjj29kea9p5mPkDPgv4Dz9qIAGNUwDpIBV5rJA7ICoyVTUotvZMW5AAZCN0fkRykbzmUntTokR0KJToquWRF3n9OMfo6vSDGEOtEU6PrqnvmXNMQJaZpoLP4Fx-hYxi3msJSYJhanfvHY03GXvMt9dAOdYykL0jhtlryrQrObywn5HtxQ8PRDj8njn98P6xt29_f6dn15x7wCNTMhDQdUvehMq1plOjABUKu214JzbpRA4BhE45TQCE3omoYLDR144_vWyWNyvu_d5vRvwTLbsd6Nw-AmTEuxoMGA7ESjqhX2Vp9TKRmD3eY4uryzwO07WbsnaytZ-07Wypr5-VG_PI3YfyU-UVaD2BtKXU3PmO0mLXmqL_-n9Q2tSoK7</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1618139254</pqid></control><display><type>article</type><title>Protective effect of CDP-choline on ischemia-reperfusion-induced myocardial tissue injury in rats</title><source>Springer Nature</source><creator>Coskun, C. ; Avci, B. ; Yalcin, M. ; Yermezler, A. ; Yilmaz, M. S. ; Savci, V.</creator><creatorcontrib>Coskun, C. ; Avci, B. ; Yalcin, M. ; Yermezler, A. ; Yilmaz, M. S. ; Savci, V.</creatorcontrib><description>Background
CDP-choline exerts tissue protective effect in several ischemic conditions. Recently we have reported that the drug prevents cardiac arrhythmias and improves survival rate in short-term myocardial ischemia reperfusion in rats.
Aim
In the current study, we determined the effect of intravenously administered CDP-choline on myocardial tissue injury induced by 30-min ischemia followed by 3-h reperfusion in anesthetized rats.
Methods
Myocardial ischemia was produced by ligature of the left main coronary artery. CDP-choline (100–500 mg/kg) was intravenously injected in the middle of the ischemic period. Cardiovascular parameters were recorded through the experimental period. At the end of the reperfusion period, the hearts of the animals were removed and stained for the investigation of tissue necrosis and apoptosis. The infarct size was evaluated as the ratio of the infarct area to the risk area. Apoptotic activation was assessed by TUNEL assay. Also the blood samples of rats were collected for the measurement of M30–M65, ADMA, homocysteine, and lactate levels.
Results
Ischemia/reperfusion caused serious injury in myocardium, increased blood ADMA and lactate levels without influencing other parameters. CDP-choline significantly reduced the infarct size and the number of apoptotic cells in the risk area. Blood pressure increased after CDP-choline injection; however, it returned back to the basal levels before the onset of reperfusion. CDP-choline failed to alter any other measured parameters.
Conclusion
The present results demonstrate that intravenously administered CDP-choline is able to protect myocardium from injury induced by long-term coronary occlusion–reperfusion in rats. The inhibition of apoptosis by the drug may contribute to its protective effect. But neither the increase in blood pressure in response to CDP-choline injection nor changes in plasma ADMA concentration appear to mediate the attenuation of the myocardial injury.</description><identifier>ISSN: 0021-1265</identifier><identifier>EISSN: 1863-4362</identifier><identifier>DOI: 10.1007/s11845-013-1046-3</identifier><identifier>PMID: 24293295</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Animals ; Apoptosis - drug effects ; Arginine - analogs & derivatives ; Arginine - blood ; Blood Pressure ; Cytidine Diphosphate Choline - therapeutic use ; Family Medicine ; General Practice ; Homocysteine - blood ; Internal Medicine ; Lactic Acid - blood ; Male ; Medicine ; Medicine & Public Health ; Myocardial Reperfusion Injury - pathology ; Myocardial Reperfusion Injury - prevention & control ; Myocardium - pathology ; Necrosis ; Original Article ; Rats ; Rats, Wistar</subject><ispartof>Irish journal of medical science, 2014-12, Vol.183 (4), p.539-548</ispartof><rights>Royal Academy of Medicine in Ireland 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-23801e4d29874748918f1e647d62000842e10ef25a426e15f955026191c8cd7a3</citedby><cites>FETCH-LOGICAL-c414t-23801e4d29874748918f1e647d62000842e10ef25a426e15f955026191c8cd7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27900,27901</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24293295$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coskun, C.</creatorcontrib><creatorcontrib>Avci, B.</creatorcontrib><creatorcontrib>Yalcin, M.</creatorcontrib><creatorcontrib>Yermezler, A.</creatorcontrib><creatorcontrib>Yilmaz, M. S.</creatorcontrib><creatorcontrib>Savci, V.</creatorcontrib><title>Protective effect of CDP-choline on ischemia-reperfusion-induced myocardial tissue injury in rats</title><title>Irish journal of medical science</title><addtitle>Ir J Med Sci</addtitle><addtitle>Ir J Med Sci</addtitle><description>Background
CDP-choline exerts tissue protective effect in several ischemic conditions. Recently we have reported that the drug prevents cardiac arrhythmias and improves survival rate in short-term myocardial ischemia reperfusion in rats.
Aim
In the current study, we determined the effect of intravenously administered CDP-choline on myocardial tissue injury induced by 30-min ischemia followed by 3-h reperfusion in anesthetized rats.
Methods
Myocardial ischemia was produced by ligature of the left main coronary artery. CDP-choline (100–500 mg/kg) was intravenously injected in the middle of the ischemic period. Cardiovascular parameters were recorded through the experimental period. At the end of the reperfusion period, the hearts of the animals were removed and stained for the investigation of tissue necrosis and apoptosis. The infarct size was evaluated as the ratio of the infarct area to the risk area. Apoptotic activation was assessed by TUNEL assay. Also the blood samples of rats were collected for the measurement of M30–M65, ADMA, homocysteine, and lactate levels.
Results
Ischemia/reperfusion caused serious injury in myocardium, increased blood ADMA and lactate levels without influencing other parameters. CDP-choline significantly reduced the infarct size and the number of apoptotic cells in the risk area. Blood pressure increased after CDP-choline injection; however, it returned back to the basal levels before the onset of reperfusion. CDP-choline failed to alter any other measured parameters.
Conclusion
The present results demonstrate that intravenously administered CDP-choline is able to protect myocardium from injury induced by long-term coronary occlusion–reperfusion in rats. The inhibition of apoptosis by the drug may contribute to its protective effect. But neither the increase in blood pressure in response to CDP-choline injection nor changes in plasma ADMA concentration appear to mediate the attenuation of the myocardial injury.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Arginine - analogs & derivatives</subject><subject>Arginine - blood</subject><subject>Blood Pressure</subject><subject>Cytidine Diphosphate Choline - therapeutic use</subject><subject>Family Medicine</subject><subject>General Practice</subject><subject>Homocysteine - blood</subject><subject>Internal Medicine</subject><subject>Lactic Acid - blood</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Myocardial Reperfusion Injury - pathology</subject><subject>Myocardial Reperfusion Injury - prevention & control</subject><subject>Myocardium - pathology</subject><subject>Necrosis</subject><subject>Original Article</subject><subject>Rats</subject><subject>Rats, Wistar</subject><issn>0021-1265</issn><issn>1863-4362</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kEFP3DAQhS3UCrbAD-BS-cjFxWM7jnNESwtISOUAZ8s4Y_Aqibd2grT_HqMFjj29kea9p5mPkDPgv4Dz9qIAGNUwDpIBV5rJA7ICoyVTUotvZMW5AAZCN0fkRykbzmUntTokR0KJToquWRF3n9OMfo6vSDGEOtEU6PrqnvmXNMQJaZpoLP4Fx-hYxi3msJSYJhanfvHY03GXvMt9dAOdYykL0jhtlryrQrObywn5HtxQ8PRDj8njn98P6xt29_f6dn15x7wCNTMhDQdUvehMq1plOjABUKu214JzbpRA4BhE45TQCE3omoYLDR144_vWyWNyvu_d5vRvwTLbsd6Nw-AmTEuxoMGA7ESjqhX2Vp9TKRmD3eY4uryzwO07WbsnaytZ-07Wypr5-VG_PI3YfyU-UVaD2BtKXU3PmO0mLXmqL_-n9Q2tSoK7</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Coskun, C.</creator><creator>Avci, B.</creator><creator>Yalcin, M.</creator><creator>Yermezler, A.</creator><creator>Yilmaz, M. S.</creator><creator>Savci, V.</creator><general>Springer London</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20141201</creationdate><title>Protective effect of CDP-choline on ischemia-reperfusion-induced myocardial tissue injury in rats</title><author>Coskun, C. ; Avci, B. ; Yalcin, M. ; Yermezler, A. ; Yilmaz, M. S. ; Savci, V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-23801e4d29874748918f1e647d62000842e10ef25a426e15f955026191c8cd7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Arginine - analogs & derivatives</topic><topic>Arginine - blood</topic><topic>Blood Pressure</topic><topic>Cytidine Diphosphate Choline - therapeutic use</topic><topic>Family Medicine</topic><topic>General Practice</topic><topic>Homocysteine - blood</topic><topic>Internal Medicine</topic><topic>Lactic Acid - blood</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Myocardial Reperfusion Injury - pathology</topic><topic>Myocardial Reperfusion Injury - prevention & control</topic><topic>Myocardium - pathology</topic><topic>Necrosis</topic><topic>Original Article</topic><topic>Rats</topic><topic>Rats, Wistar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coskun, C.</creatorcontrib><creatorcontrib>Avci, B.</creatorcontrib><creatorcontrib>Yalcin, M.</creatorcontrib><creatorcontrib>Yermezler, A.</creatorcontrib><creatorcontrib>Yilmaz, M. S.</creatorcontrib><creatorcontrib>Savci, V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Irish journal of medical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coskun, C.</au><au>Avci, B.</au><au>Yalcin, M.</au><au>Yermezler, A.</au><au>Yilmaz, M. S.</au><au>Savci, V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effect of CDP-choline on ischemia-reperfusion-induced myocardial tissue injury in rats</atitle><jtitle>Irish journal of medical science</jtitle><stitle>Ir J Med Sci</stitle><addtitle>Ir J Med Sci</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>183</volume><issue>4</issue><spage>539</spage><epage>548</epage><pages>539-548</pages><issn>0021-1265</issn><eissn>1863-4362</eissn><abstract>Background
CDP-choline exerts tissue protective effect in several ischemic conditions. Recently we have reported that the drug prevents cardiac arrhythmias and improves survival rate in short-term myocardial ischemia reperfusion in rats.
Aim
In the current study, we determined the effect of intravenously administered CDP-choline on myocardial tissue injury induced by 30-min ischemia followed by 3-h reperfusion in anesthetized rats.
Methods
Myocardial ischemia was produced by ligature of the left main coronary artery. CDP-choline (100–500 mg/kg) was intravenously injected in the middle of the ischemic period. Cardiovascular parameters were recorded through the experimental period. At the end of the reperfusion period, the hearts of the animals were removed and stained for the investigation of tissue necrosis and apoptosis. The infarct size was evaluated as the ratio of the infarct area to the risk area. Apoptotic activation was assessed by TUNEL assay. Also the blood samples of rats were collected for the measurement of M30–M65, ADMA, homocysteine, and lactate levels.
Results
Ischemia/reperfusion caused serious injury in myocardium, increased blood ADMA and lactate levels without influencing other parameters. CDP-choline significantly reduced the infarct size and the number of apoptotic cells in the risk area. Blood pressure increased after CDP-choline injection; however, it returned back to the basal levels before the onset of reperfusion. CDP-choline failed to alter any other measured parameters.
Conclusion
The present results demonstrate that intravenously administered CDP-choline is able to protect myocardium from injury induced by long-term coronary occlusion–reperfusion in rats. The inhibition of apoptosis by the drug may contribute to its protective effect. But neither the increase in blood pressure in response to CDP-choline injection nor changes in plasma ADMA concentration appear to mediate the attenuation of the myocardial injury.</abstract><cop>London</cop><pub>Springer London</pub><pmid>24293295</pmid><doi>10.1007/s11845-013-1046-3</doi><tpages>10</tpages></addata></record> |
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| ispartof | Irish journal of medical science, 2014-12, Vol.183 (4), p.539-548 |
| issn | 0021-1265 1863-4362 |
| language | eng |
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| source | Springer Nature |
| subjects | Animals Apoptosis - drug effects Arginine - analogs & derivatives Arginine - blood Blood Pressure Cytidine Diphosphate Choline - therapeutic use Family Medicine General Practice Homocysteine - blood Internal Medicine Lactic Acid - blood Male Medicine Medicine & Public Health Myocardial Reperfusion Injury - pathology Myocardial Reperfusion Injury - prevention & control Myocardium - pathology Necrosis Original Article Rats Rats, Wistar |
| title | Protective effect of CDP-choline on ischemia-reperfusion-induced myocardial tissue injury in rats |
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