Oligo-microarray analysis and identification of stress-immune response genes from manila clam (Ruditapes philippinarum) exposure to heat and cold stresses

• Published in: Molecular biology reports 2014-10, Vol.41 (10), p.6457-6473
• Main Authors: Menike, Udeni, Lee, Youngdeuk, Oh, Chulhong, Wickramaarachchi, W. D. N, Premachandra, H. K. A, Park, Se Chang, Lee, Jehee, De Zoysa, Mahanama
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Animals; antioxidants; apoptosis; Biomedical and Life Sciences; Bivalvia - genetics; Bivalvia - immunology; cDNA libraries; clams; Cluster Analysis; Cold; cold stress; Cold-Shock Response - genetics; complementary DNA; Crustaceans; cytokines; enzymes; gas exchange; gene expression; Gene Expression Profiling; Gene Expression Regulation; Gene-Environment Interaction; Genes; gills; Gills - metabolism; Heat; heat shock proteins; heat stress; Heat-Shock Response - genetics; Histology; hypertrophy; hypoxia; Immune system; Life Sciences; microarray technology; Morphology; necrosis; regulatory proteins; Ruditapes philippinarum; sequence analysis; Stress analysis; stress response; transcription factors; Transcriptome
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-014-3529-3

Thermal stress regulates the complex system of gene expression and downstream biochemical and physiological responses in aquatic species. To identify genes involved in heat stress responses in manila clam (Ruditapes philippinarum), microarray analysis was conducted using clam transcriptome generated by pyrosequencing of cDNA library. Manila clams were exposed to heat (30 ± 1 °C) and cold (4 ± 1 °C) stresses and compared with control animals (18 ± 1 °C). Heat stressed animals have changed greater number of transcripts (8,306) than cold stress (7,573). Results of both heat and cold exposure has shown that over 2-fold up-regulated or down regulated (>2-or