NHE1 gene associated with avian leukosis virus subgroup J infection in chicken

• Published in: Molecular biology reports 2014-10, Vol.41 (10), p.6519-6524
• Main Authors: Chen, Biao, Pan, Weiling, Zhang, Liangyu, Liu, Jing, Ouyang, Hongjia, Nie, Qinghua, Zhang, Xiquan
• Format: Article
• Language: English
• Subjects: Alleles; Animal Anatomy; Animal Biochemistry; Animals; Avian Leukosis - genetics; Avian leukosis virus; Avian Leukosis Virus - classification; Avian Leukosis Virus - genetics; Avian Leukosis Virus - immunology; Biomedical and Life Sciences; Birds; Chickens; Female; Gene Expression; Genes; Genetic Predisposition to Disease; Genotype; Histology; Infections; Life Sciences; Morphology; Polymorphism, Single Nucleotide; Poultry; Sodium-Hydrogen Exchangers - genetics; Viruses
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-014-3535-5

As a kind of binding protein, the type 1 Na + /H + exchanger (NHE1) is a receptor for the highly pathogenic Avian leukosis viruses-J subgroup (ALV-J) in chicken. In order to investigate the potential effect of chicken NHE1 gene on leukosis, we compared its expression between ALV-J-affected and -unaffected chicken, screened variations across the whole gene, and then performed association analysis with ALV-J affected/unaffected trait in three un-related chicken populations. We found that the NHE1 gene expressed in four immune tissues including spleen, bursa fabricius, liver, and thymus, and its expression was significantly up-regulated in liver and thymus of ALV-J-affected chickens (with leukosis phenotype) compared to -unaffected ones (ALV-J-negative controls). Thirty-six single nucleotide polymorphisms (SNP) were identified in a 6,105 bp region of the chicken NHE1 gene, giving rise to every 170 bp per SNP. Two SNP of g.4405A>G and g.5886C>G were genotyped with PCR-RFLP method. Results showed that g.4405A>G was significantly associated ( P  G was significantly associated ( P