Zerumbone Suppresses IL‐1β‐induced Cell Migration and Invasion by Inhibiting IL‐8 and MMP‐3 Expression in Human Triple‐negative Breast Cancer Cells

• Published in: Phytotherapy research 2014-11, Vol.28 (11), p.1654-1660
• Main Authors: Han, Jeonghun, Bae, Soo Youn, Oh, Soo‐Jin, Lee, Jeongmin, Lee, Jun Ho, Lee, Hyun‐chul, Lee, Se Kyung, Kil, Won Ho, Kim, Seok Won, Nam, Seok Jin, Kim, Sangmin, Lee, Jeong Eon
• Format: Article
• Language: English
• Subjects: breast neoplasms; cell invasion; Cell Line, Tumor; cell movement; Cell Movement - drug effects; drug therapy; Female; Gene Expression Regulation, Neoplastic; Humans; IL-1β; IL-8; inflammation; interleukin-1beta; Interleukin-1beta - pharmacology; Interleukin-8 - metabolism; Matrix Metalloproteinase 3 - metabolism; messenger RNA; metastasis; MMP-3; Neoplasm Invasiveness; patients; polymerase chain reaction; Sesquiterpenes - pharmacology; Triple Negative Breast Neoplasms - metabolism; Triple Negative Breast Neoplasms - pathology; triple-negative breast cancer; Western blotting; zerumbone
• ISSN: 0951-418X; 1099-1573
• DOI: 10.1002/ptr.5178

Inflammation is a key regulatory process in cancer development. Prolonged exposure of breast tumor cells to inflammatory cytokines leads to epithelial‐mesenchymal transition, which is the principal mechanism involved in metastasis and tumor invasion. Interleukin (IL)‐1β is a major inflammatory cytokine in a variety of tumors. To date, the regulatory mechanism of IL‐1β‐induced cell migration and invasion has not been fully elucidated. Here, we investigated the effect of zerumbone (ZER) on IL‐1β‐induced cell migration and invasion in breast cancer cells. The levels of IL‐8 and matrix metalloproteinase (MMP)‐3 mRNA were analyzed by real‐time polymerase chain reaction. The levels of secreted IL‐8 and MMP‐3 protein were analyzed by enzyme‐linked immunosorbent assay and western blot analysis, respectively. Cell invasion and migration was detected by Boyden chamber assay. The levels of IL‐8 and MMP‐3 expression were significantly increased by IL‐1β treatment in Hs578T and MDA‐MB231 cells. On the other hand, IL‐1β‐induced IL‐8 and MMP‐3 expression was decreased by ZER. Finally, IL‐1β‐induced cell migration and invasion were decreased by ZER in Hs578T and MDA‐MB231 cells. ZER suppresses IL‐1β‐induced cell migration and invasion by inhibiting IL‐8 expression and MMP‐3 expression in TNBC cells. ZER could be a promising therapeutic drug for treatment of triple‐negative breast cancer patients. Copyright © 2014 John Wiley & Sons, Ltd.