Optimizing Cultivation Strategies for Robust Algal Growth and Consequent Removal of Inorganic Nutrients in Pretreated Livestock Effluent

Dilution was employed as a pretreatment strategy to increase light transmittance and decrease ammonia toxicity in piggery effluent prior to the cultivation of microalgae. The dilution effect was quantitatively determined based on both the maximum specific nutrient consumption rate and the maximum gr...

Full description

Saved in:
Bibliographic Details
Published in:Applied biochemistry and biotechnology 2014-10, Vol.174 (4), p.1668-1682
Main Authors: Kim, Hyun-Chul, Choi, Wook Jin, Ryu, Jun Hee, Maeng, Sung Kyu, Kim, Han Soo, Lee, Byung-Chan, Song, Kyung Guen
Format: Article
Language:English
Subjects:
Algae
Algal growth
ammonia
Animal wastes
Animals
Biochemistry
Biological and medical sciences
Biomass
biomass production
Bioremediation
Biotechnology
Chemistry
Chemistry and Materials Science
Cultivation
Effluents
Fundamental and applied biological sciences. Psychology
harvesting
Livestock
Medical Waste Disposal - methods
Microalgae
Microalgae - growth & development
nitrogen content
Nutrient removal
Nutrients
Scenedesmus - growth & development
Scenedesmus quadricauda
Toxicity
transmittance
Waste Water - microbiology
wastewater
Water Purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c492t-d140e47c848bc047a10aab8a94338e42ba95fe2291b83ff8167bf87f940ec7de3
cites cdi_FETCH-LOGICAL-c492t-d140e47c848bc047a10aab8a94338e42ba95fe2291b83ff8167bf87f940ec7de3
container_end_page 1682
container_issue 4
container_start_page 1668
container_title Applied biochemistry and biotechnology
container_volume 174
creator Kim, Hyun-Chul
Choi, Wook Jin
Ryu, Jun Hee
Maeng, Sung Kyu
Kim, Han Soo
Lee, Byung-Chan
Song, Kyung Guen
description Dilution was employed as a pretreatment strategy to increase light transmittance and decrease ammonia toxicity in piggery effluent prior to the cultivation of microalgae. The dilution effect was quantitatively determined based on both the maximum specific nutrient consumption rate and the maximum growth coefficient to minimize the usage of diluent. The biomass productivity of microalgae was also evaluated to select the best species among the five different candidates examined. A 20-fold dilution of piggery wastewater resulted in decreased chromaticity (584 mg Pt-Co L⁻¹) and total nitrogen (76 mg L⁻¹), on which the microalgae cultivation was more effective for an algal growth compared to the other dilution factors. If the initial cell concentration of Scenedesmus quadricauda increased, the production of biomass tended to improve. Robust growth and harvesting of S. quadricauda were achieved, and the associated consistent removal of inorganic nutrients was accomplished during the semi-continuous cultivation of the best species.
doi_str_mv 10.1007/s12010-014-1145-2
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1618156730</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3445954771</sourcerecordid><originalsourceid>FETCH-LOGICAL-c492t-d140e47c848bc047a10aab8a94338e42ba95fe2291b83ff8167bf87f940ec7de3</originalsourceid><addsrcrecordid>eNp9kc1u1DAUhS0EokPhAdiAJYTEJuDr_DnLalRKpRFFLV1bjmMHl4w92M4geAIemzvK8CMWeOPF-c7xsQ4hT4G9BsbaNwk4A1YwqAqAqi74PbKCuu4Kxju4T1aMt2XBuehOyKOU7hgDLur2ITnhNZSiYbAiP6522W3dd-dHup6n7PYqu-DpTY4qm9GZRG2I9Dr0c8r0bBrVRC9i-Jo_UeUHug4-mS-z8Zlem23YoxosvfQhjso7Td_POTpUE3WefogmR4OxA924vUk56M_03Nrp4H9MHlg1JfPkeJ-S27fnH9fvis3VxeX6bFPoquO5GKBipmq1qESvWdUqYEr1QnVVWQpT8V51tTUc_9-L0loBTdtb0doObbodTHlKXi25uxiweMpy65I206S8CXOS0ICAumlLhuiLf9C7MEeP7SQSDTaBskEKFkrHkFI0Vu6i26r4TQKTh5nkMpPEmeRhJsnR8-yYPPdbM_x2_NoFgZdHQCWtJhuV1y794YQQXPAOOb5wCSU_mvhXxf-8_nwxWRWkGiMG394gVDM8NW-68icyMrS1</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1566140136</pqid></control><display><type>article</type><title>Optimizing Cultivation Strategies for Robust Algal Growth and Consequent Removal of Inorganic Nutrients in Pretreated Livestock Effluent</title><source>Springer Nature</source><creator>Kim, Hyun-Chul ; Choi, Wook Jin ; Ryu, Jun Hee ; Maeng, Sung Kyu ; Kim, Han Soo ; Lee, Byung-Chan ; Song, Kyung Guen</creator><creatorcontrib>Kim, Hyun-Chul ; Choi, Wook Jin ; Ryu, Jun Hee ; Maeng, Sung Kyu ; Kim, Han Soo ; Lee, Byung-Chan ; Song, Kyung Guen</creatorcontrib><description>Dilution was employed as a pretreatment strategy to increase light transmittance and decrease ammonia toxicity in piggery effluent prior to the cultivation of microalgae. The dilution effect was quantitatively determined based on both the maximum specific nutrient consumption rate and the maximum growth coefficient to minimize the usage of diluent. The biomass productivity of microalgae was also evaluated to select the best species among the five different candidates examined. A 20-fold dilution of piggery wastewater resulted in decreased chromaticity (584 mg Pt-Co L⁻¹) and total nitrogen (76 mg L⁻¹), on which the microalgae cultivation was more effective for an algal growth compared to the other dilution factors. If the initial cell concentration of Scenedesmus quadricauda increased, the production of biomass tended to improve. Robust growth and harvesting of S. quadricauda were achieved, and the associated consistent removal of inorganic nutrients was accomplished during the semi-continuous cultivation of the best species.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-014-1145-2</identifier><identifier>PMID: 25138601</identifier><identifier>CODEN: ABIBDL</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Algae ; Algal growth ; ammonia ; Animal wastes ; Animals ; Biochemistry ; Biological and medical sciences ; Biomass ; biomass production ; Bioremediation ; Biotechnology ; Chemistry ; Chemistry and Materials Science ; Cultivation ; Effluents ; Fundamental and applied biological sciences. Psychology ; harvesting ; Livestock ; Medical Waste Disposal - methods ; Microalgae ; Microalgae - growth &amp; development ; nitrogen content ; Nutrient removal ; Nutrients ; Scenedesmus - growth &amp; development ; Scenedesmus quadricauda ; Toxicity ; transmittance ; Waste Water - microbiology ; wastewater ; Water Purification</subject><ispartof>Applied biochemistry and biotechnology, 2014-10, Vol.174 (4), p.1668-1682</ispartof><rights>Springer Science+Business Media New York 2014</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-d140e47c848bc047a10aab8a94338e42ba95fe2291b83ff8167bf87f940ec7de3</citedby><cites>FETCH-LOGICAL-c492t-d140e47c848bc047a10aab8a94338e42ba95fe2291b83ff8167bf87f940ec7de3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=28882829$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25138601$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Hyun-Chul</creatorcontrib><creatorcontrib>Choi, Wook Jin</creatorcontrib><creatorcontrib>Ryu, Jun Hee</creatorcontrib><creatorcontrib>Maeng, Sung Kyu</creatorcontrib><creatorcontrib>Kim, Han Soo</creatorcontrib><creatorcontrib>Lee, Byung-Chan</creatorcontrib><creatorcontrib>Song, Kyung Guen</creatorcontrib><title>Optimizing Cultivation Strategies for Robust Algal Growth and Consequent Removal of Inorganic Nutrients in Pretreated Livestock Effluent</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Dilution was employed as a pretreatment strategy to increase light transmittance and decrease ammonia toxicity in piggery effluent prior to the cultivation of microalgae. The dilution effect was quantitatively determined based on both the maximum specific nutrient consumption rate and the maximum growth coefficient to minimize the usage of diluent. The biomass productivity of microalgae was also evaluated to select the best species among the five different candidates examined. A 20-fold dilution of piggery wastewater resulted in decreased chromaticity (584 mg Pt-Co L⁻¹) and total nitrogen (76 mg L⁻¹), on which the microalgae cultivation was more effective for an algal growth compared to the other dilution factors. If the initial cell concentration of Scenedesmus quadricauda increased, the production of biomass tended to improve. Robust growth and harvesting of S. quadricauda were achieved, and the associated consistent removal of inorganic nutrients was accomplished during the semi-continuous cultivation of the best species.</description><subject>Algae</subject><subject>Algal growth</subject><subject>ammonia</subject><subject>Animal wastes</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>biomass production</subject><subject>Bioremediation</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cultivation</subject><subject>Effluents</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>harvesting</subject><subject>Livestock</subject><subject>Medical Waste Disposal - methods</subject><subject>Microalgae</subject><subject>Microalgae - growth &amp; development</subject><subject>nitrogen content</subject><subject>Nutrient removal</subject><subject>Nutrients</subject><subject>Scenedesmus - growth &amp; development</subject><subject>Scenedesmus quadricauda</subject><subject>Toxicity</subject><subject>transmittance</subject><subject>Waste Water - microbiology</subject><subject>wastewater</subject><subject>Water Purification</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kc1u1DAUhS0EokPhAdiAJYTEJuDr_DnLalRKpRFFLV1bjmMHl4w92M4geAIemzvK8CMWeOPF-c7xsQ4hT4G9BsbaNwk4A1YwqAqAqi74PbKCuu4Kxju4T1aMt2XBuehOyKOU7hgDLur2ITnhNZSiYbAiP6522W3dd-dHup6n7PYqu-DpTY4qm9GZRG2I9Dr0c8r0bBrVRC9i-Jo_UeUHug4-mS-z8Zlem23YoxosvfQhjso7Td_POTpUE3WefogmR4OxA924vUk56M_03Nrp4H9MHlg1JfPkeJ-S27fnH9fvis3VxeX6bFPoquO5GKBipmq1qESvWdUqYEr1QnVVWQpT8V51tTUc_9-L0loBTdtb0doObbodTHlKXi25uxiweMpy65I206S8CXOS0ICAumlLhuiLf9C7MEeP7SQSDTaBskEKFkrHkFI0Vu6i26r4TQKTh5nkMpPEmeRhJsnR8-yYPPdbM_x2_NoFgZdHQCWtJhuV1y794YQQXPAOOb5wCSU_mvhXxf-8_nwxWRWkGiMG394gVDM8NW-68icyMrS1</recordid><startdate>20141001</startdate><enddate>20141001</enddate><creator>Kim, Hyun-Chul</creator><creator>Choi, Wook Jin</creator><creator>Ryu, Jun Hee</creator><creator>Maeng, Sung Kyu</creator><creator>Kim, Han Soo</creator><creator>Lee, Byung-Chan</creator><creator>Song, Kyung Guen</creator><general>Springer-Verlag</general><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7QO</scope><scope>F1W</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>20141001</creationdate><title>Optimizing Cultivation Strategies for Robust Algal Growth and Consequent Removal of Inorganic Nutrients in Pretreated Livestock Effluent</title><author>Kim, Hyun-Chul ; Choi, Wook Jin ; Ryu, Jun Hee ; Maeng, Sung Kyu ; Kim, Han Soo ; Lee, Byung-Chan ; Song, Kyung Guen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-d140e47c848bc047a10aab8a94338e42ba95fe2291b83ff8167bf87f940ec7de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Algae</topic><topic>Algal growth</topic><topic>ammonia</topic><topic>Animal wastes</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomass</topic><topic>biomass production</topic><topic>Bioremediation</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cultivation</topic><topic>Effluents</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>harvesting</topic><topic>Livestock</topic><topic>Medical Waste Disposal - methods</topic><topic>Microalgae</topic><topic>Microalgae - growth &amp; development</topic><topic>nitrogen content</topic><topic>Nutrient removal</topic><topic>Nutrients</topic><topic>Scenedesmus - growth &amp; development</topic><topic>Scenedesmus quadricauda</topic><topic>Toxicity</topic><topic>transmittance</topic><topic>Waste Water - microbiology</topic><topic>wastewater</topic><topic>Water Purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Hyun-Chul</creatorcontrib><creatorcontrib>Choi, Wook Jin</creatorcontrib><creatorcontrib>Ryu, Jun Hee</creatorcontrib><creatorcontrib>Maeng, Sung Kyu</creatorcontrib><creatorcontrib>Kim, Han Soo</creatorcontrib><creatorcontrib>Lee, Byung-Chan</creatorcontrib><creatorcontrib>Song, Kyung Guen</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Hyun-Chul</au><au>Choi, Wook Jin</au><au>Ryu, Jun Hee</au><au>Maeng, Sung Kyu</au><au>Kim, Han Soo</au><au>Lee, Byung-Chan</au><au>Song, Kyung Guen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimizing Cultivation Strategies for Robust Algal Growth and Consequent Removal of Inorganic Nutrients in Pretreated Livestock Effluent</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2014-10-01</date><risdate>2014</risdate><volume>174</volume><issue>4</issue><spage>1668</spage><epage>1682</epage><pages>1668-1682</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><coden>ABIBDL</coden><abstract>Dilution was employed as a pretreatment strategy to increase light transmittance and decrease ammonia toxicity in piggery effluent prior to the cultivation of microalgae. The dilution effect was quantitatively determined based on both the maximum specific nutrient consumption rate and the maximum growth coefficient to minimize the usage of diluent. The biomass productivity of microalgae was also evaluated to select the best species among the five different candidates examined. A 20-fold dilution of piggery wastewater resulted in decreased chromaticity (584 mg Pt-Co L⁻¹) and total nitrogen (76 mg L⁻¹), on which the microalgae cultivation was more effective for an algal growth compared to the other dilution factors. If the initial cell concentration of Scenedesmus quadricauda increased, the production of biomass tended to improve. Robust growth and harvesting of S. quadricauda were achieved, and the associated consistent removal of inorganic nutrients was accomplished during the semi-continuous cultivation of the best species.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>25138601</pmid><doi>10.1007/s12010-014-1145-2</doi><tpages>15</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0273-2289
ispartof Applied biochemistry and biotechnology, 2014-10, Vol.174 (4), p.1668-1682
issn 0273-2289
1559-0291
language eng
recordid cdi_proquest_miscellaneous_1618156730
source Springer Nature
subjects Algae
Algal growth
ammonia
Animal wastes
Animals
Biochemistry
Biological and medical sciences
Biomass
biomass production
Bioremediation
Biotechnology
Chemistry
Chemistry and Materials Science
Cultivation
Effluents
Fundamental and applied biological sciences. Psychology
harvesting
Livestock
Medical Waste Disposal - methods
Microalgae
Microalgae - growth & development
nitrogen content
Nutrient removal
Nutrients
Scenedesmus - growth & development
Scenedesmus quadricauda
Toxicity
transmittance
Waste Water - microbiology
wastewater
Water Purification
title Optimizing Cultivation Strategies for Robust Algal Growth and Consequent Removal of Inorganic Nutrients in Pretreated Livestock Effluent
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T09%3A08%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Optimizing%20Cultivation%20Strategies%20for%20Robust%20Algal%20Growth%20and%20Consequent%20Removal%20of%20Inorganic%20Nutrients%20in%20Pretreated%20Livestock%20Effluent&rft.jtitle=Applied%20biochemistry%20and%20biotechnology&rft.au=Kim,%20Hyun-Chul&rft.date=2014-10-01&rft.volume=174&rft.issue=4&rft.spage=1668&rft.epage=1682&rft.pages=1668-1682&rft.issn=0273-2289&rft.eissn=1559-0291&rft.coden=ABIBDL&rft_id=info:doi/10.1007/s12010-014-1145-2&rft_dat=%3Cproquest_cross%3E3445954771%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c492t-d140e47c848bc047a10aab8a94338e42ba95fe2291b83ff8167bf87f940ec7de3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1566140136&rft_id=info:pmid/25138601&rfr_iscdi=true