Diatom-Based Label-Free Optical Biosensor for Biomolecules

• Published in: Applied biochemistry and biotechnology 2014-10, Vol.174 (3), p.1166-1173
• Main Authors: Viji, S, Anbazhagi, M, Ponpandian, N, Mangalaraj, D, Jeyanthi, S, Santhanam, P, Devi, A. Shenbaga, Viswanathan, C
• Format: Article
• Language: English
• Subjects: Algae; Amphora; Animals; Bacillariophyceae; Biochemistry; Biological and medical sciences; Biosensing Techniques; Biosensors; Biotechnology; bovine serum albumin; Cattle; Chemistry; Chemistry and Materials Science; detection limit; Diatoms - chemistry; Emissions; Fourier transform infrared spectroscopy; Fourier transforms; Fundamental and applied biological sciences. Psychology; Luminescence; Methods. Procedures. Technologies; Microscopy, Electron, Scanning; Molecules; Nanoscience; Nanostructures - chemistry; Nanotechnology; Porosity; Propylamines; Scanning electron microscopy; Serum Albumin, Bovine - chemistry; Serum Albumin, Bovine - isolation & purification; Silanes - chemistry; Silicon Dioxide - chemistry; Various methods and equipments
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-014-1040-x

Diatoms are unicellular algae, which fabricates ornate biosilica shells called frustules that possess a surface rich in reactive silanol (Si–OH) groups. The intrinsic patterned porous structure of diatom frustules at nanoscale can be exploited in the effective detection of biomolecules. In this study, the frustules of a specific diatom Amphora sp. has been functionalized to detect bovine serum albumin (BSA). The functionalization of the diatom frustule substrate is achieved by using 3-aminopropyltriethoxysilane (APES). The field emission scanning electron microscopy (FESEM) results showed an ornately patterned surface of the frustule valve ordered at nanoscale. The Fourier transform infrared (FTIR) spectra confirmed the N–H bending and stretching of the amine group after amine functionalization. The emission peaks in the photoluminescence (PL) spectra of the amine-functionalized diatom biosilica selectively enhanced the intensity by a factor of ten when compared to that of a bare diatom biosilica. The result showed a significant quenching of PL intensity of BSA at around 445 nm due to the interaction of amine-functionalized diatom–BSA protein complex. The detection limit was found to be 3 × 10⁻⁵ M of BSA protein. Hence, the study proves that the functionalized frustule of Amphora sp. is an effective quantitative analytical tool for optical label-free biosensing applications.