Molecular cloning, sequencing, and physiological characterization of the qox operon from Bacillus subtilis encoding the aa sub(3)-600 quinol oxidase

Bacillus subtilis contains two aa sub(3)-type terminal oxidases (caa sub(3)-605 and aa sub(3)-600) catalyzing cytochrome c and quinol oxidation, respectively, with the concomitant reduction of O sub(2) to H sub(2)O. Previous studies characterized only the structural genes of caa sub(3)-605 oxidase....

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Bibliographic Details
Published in:The Journal of biological chemistry 1992-01, Vol.267 (15), p.10225-10231
Main Authors: Santana, M, Kunst, F, Hullo, M F, Rapoport, G, Danchin, A, Glaser, P
Format: Article
Language:English
Subjects:
amino acid sequence
Bacillus subtilis
nucleotide sequence
operons
predictions
qox operon
quinol oxidase
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Summary:Bacillus subtilis contains two aa sub(3)-type terminal oxidases (caa sub(3)-605 and aa sub(3)-600) catalyzing cytochrome c and quinol oxidation, respectively, with the concomitant reduction of O sub(2) to H sub(2)O. Previous studies characterized only the structural genes of caa sub(3)-605 oxidase. We isolated the genes coding for the four subunits of a B. subtilis) terminal oxidase from a genomic DNA library. These genes, named qoxA to qoxD, are organized in an operon. Examination of the deduced amino acid sequence of Qox subunits showed that this oxidase is structurally related to the large family of mitochondrial-type aa sub(3) terminal oxidases.
ISSN:0021-9258