Production of alkaline protease with Bacillus licheniformis in a controlled fed-batch process

The alkaline serine protease subtilisin Carlsberg is excreted into the medium by strains of Bacillus licheniformis or B. pumilus in the early stationary phase. A production method for alkaline serine protease with Bacillus licheniformis in a synthetic medium was developed. Employing closed-loop cont...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1991-09, Vol.35 (6), p.720-724
Main Authors: EBERHARD GIESECKE, U, BIERBAUM, G, RUDDE, H, SPOHN, U, WANDREY, C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Enzyme engineering
Fermentation
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Production of selected enzymes
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Summary:The alkaline serine protease subtilisin Carlsberg is excreted into the medium by strains of Bacillus licheniformis or B. pumilus in the early stationary phase. A production method for alkaline serine protease with Bacillus licheniformis in a synthetic medium was developed. Employing closed-loop control of oxygen, nitrogen and carbon source the pO sub(2) was held at 5%, the ammonium concentration kept below 1 mM and the glycerol concentration was maintained between 20 and 100 mM. Protease production was monitored by flow injection analysis. Thus, in a fed-batch procedure production could be increased 4.6-fold in comparison to an uncontrolled batch process.
ISSN:0175-7598
1432-0614
DOI:10.1007/BF00169884