Expression of v-src in embryonic neural retina alters cells adhesion, inhibits histogenesis, and prevents induction of glutamine synthetase

Using Rous sarcoma virus as the vector, v-src or c-src genes were introduced into 6-day chicken embryo retina tissue in organ culture and their effects on retina development were investigated. Overexpression of c-src in many of the cells had no noticeable effect on retina development. In contrast, i...

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Bibliographic Details
Published in:Molecular and cellular biology 1991-01, Vol.11 (10), p.5275-5284
Main Authors: Vardimon, L, Fox, LE, Cohen-Kupiec, R, Degenstein, L, Moscona, A A
Format: Article
Language:English
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Summary:Using Rous sarcoma virus as the vector, v-src or c-src genes were introduced into 6-day chicken embryo retina tissue in organ culture and their effects on retina development were investigated. Overexpression of c-src in many of the cells had no noticeable effect on retina development. In contrast, infection with v-src resulted in abnormal histogenesis and inhibition of differentiation. Although only a portion of the cells in infected tissue expressed the oncogene and displayed the transformation phenotype, the other cells were also hindered from becoming normally positioned and organized. Therefore, presence of oncogene-transformed cells within the tissue hindered organization and development of adjacent nontransformed cells.
ISSN:0270-7306