ATP synthesis from 2,3-diphosphoglycerate by cell-free extract of Methanobacterium thermoautotrophicum (strain ΔH)

Cell-free extracts of Methanobacterium thermoautotrophicum were found to catalyze ATP synthesis from an endogeneous substrate. Synthesis was stimulated under hydrogen atmosphere and inhibited by KCl (K sub(i) = 150 mM). Comparison of the properties of a number of cell constituents showed the endogen...

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Bibliographic Details
Published in:Archives of microbiology 1991-11, Vol.156 (6), p.491-496
Main Authors: VAN ALEBEEK, G.-J. W. M, KLAASSEN, C, KELTJENS, J. T, VAN DER DRIFT, C, VOGELS, G. D
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Methanobacterium thermoautotrophicum
Microbiology
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Summary:Cell-free extracts of Methanobacterium thermoautotrophicum were found to catalyze ATP synthesis from an endogeneous substrate. Synthesis was stimulated under hydrogen atmosphere and inhibited by KCl (K sub(i) = 150 mM). Comparison of the properties of a number of cell constituents showed the endogeneous substrate to be 2,3-diphosphoglycerate. The compound is converted into 3-phosphoglycerate, and via 2-phosphoglycerate and phosphoenolpyruvate into pyruvate, at which the latter reaction is linked with ATP synthesis.
ISSN:0302-8933
1432-072X
DOI:10.1007/BF00245397