Induction of cell fusion by a factor released by the cellular slime mold Polysphondylium pallidum

Heterokaryons and hybrid cells, which are extremely useful for research in cell biology, can be produced artificially by treating cells with either polyethylene glycol or certain inactivated viruses that alter the plasma membrane. We report here a novel cell-fusion inducing factor secreted by CK-8 s...

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Bibliographic Details
Published in:Archives of microbiology 1991-08, Vol.156 (3), p.159-162
Main Authors: MIZUTANI, A, HABATA, Y, YANAGISAWA, K
Format: Article
Language:English
Subjects:
Animal cells
Biological and medical sciences
Cell cultures. Hybridization. Fusion
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
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Summary:Heterokaryons and hybrid cells, which are extremely useful for research in cell biology, can be produced artificially by treating cells with either polyethylene glycol or certain inactivated viruses that alter the plasma membrane. We report here a novel cell-fusion inducing factor secreted by CK-8 strain cells of cellular slime mold Polysphondylium pallidum . Treatment of other strains or other species of cellular slime molds, such as NC-4 of Dictyostelium discoideum with the diluted fraction, containing molecules larger than 50 kDa, of the conditioned medium of CK-8 cell culture induces cell fusion at high frequency and produces multinucleated large cells. This cell fusion is inducible between cells of either a single strain or of two different strains of cellular slime molds.
ISSN:0302-8933
1432-072X
DOI:10.1007/BF00249108