Mitochondrially-imported cytoplasmic tRNA super(Lys)(CUU) of Saccharomyces cerevisiae : In vivo and in vitro targeting systems

The cytoplasmic tRNA super(Lys)(CUU) (tRNA sub(1) super(Lys)) is the single yeast tRNA species to be traffiked from the cytoplasm into the mitochondrial compartment of the cell. To study mechanisms of this targetting we worked out two test systems. The in vivo system based on the electroporation of...

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Bibliographic Details
Published in:Nucleic acids research 1992-01, Vol.20 (6), p.1277-1281
Main Authors: Tarassov, IA, Entelis, N S
Format: Article
Language:English
Subjects:
Saccharomyces cerevisiae
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Summary:The cytoplasmic tRNA super(Lys)(CUU) (tRNA sub(1) super(Lys)) is the single yeast tRNA species to be traffiked from the cytoplasm into the mitochondrial compartment of the cell. To study mechanisms of this targetting we worked out two test systems. The in vivo system based on the electroporation of intact yeast cells was used to introduce labelled tRNAs into the cytoplasm. All tRNA species tested were effectively introduced into the cytoplasm, but only the cytoplasmic tRNA sub(1) super(Lys) was found in the mitochondrial compartment within 1-2 hours after the electroporation procedure. The in vitro system permits specific transfer of the tRNA sub(1) super(Lys) into isolated mitochondria.
ISSN:0305-1048